Evidence map›Paper›PMID 40140541›Full record

ArticleScientific reports2025

Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork.

Warawan Eiamphungporn, Pattrathip Laohabutr, Noramon Kaewsai, Sudarat Pornsuwan, Sakda Yainoy, Chawalit Chatupheeraphat

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Rapid and specific detection ofFrontiers in veterinary science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Warawan EiamphungpornDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Pattrathip LaohabutrDepartment of Medical Technology and Clinical Pathology, Ban Pong Hospital, Ban Pong, Ratchaburi, Thailand.
Noramon KaewsaiCenter for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Salaya, Nakhon Pathom, Thailand.
Sudarat PornsuwanInternational Center for Medical and Radiological Technology, Faculty of Medical Technology, Mahidol University, Salaya, Nakhon Pathom, Thailand.
Sakda YainoyDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Chawalit ChatupheeraphatCenter for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Salaya, Nakhon Pathom, Thailand. chawalit.cha@mahidol.ac.th.

Funding

Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, Thailand RGNS 65-144
6 · The paper itself

Abstract

Streptococcus suis is a major pathogen in pigs, causing diseases like meningitis, septicemia, and pneumonia, and has become a serious zoonosis in humans, particularly in countries with intensive swine production. Human infections have risen significantly, especially in Thailand, where serotype 2 is most commonly associated with disease in both pigs and humans. Traditional methods of identifying S. suis and its serotypes, such as bacterial culture, biochemical testing, and agglutination tests, face challenges due to variability and limited antisera availability, leading to the need for alternative approaches. In this study, we developed a novel RPA-NALFIA assay targeting the recN and cps2J genes of S. suis serotype 2. This method demonstrated accurate identification without cross-reactivity among 28 other bacterial species, with a detection limit of 10

Indexed as

Nucleic Acid Amplification TechniquesPork MeatRecombinasesRed MeatStreptococcal InfectionsStreptococcus suisAnimalsFood MicrobiologySensitivity and SpecificitySerogroupSwineSwine DiseasesThailandRecombinasesFood safetyLateral flowPorkRecombinase polymerase amplificationStreptococcus suis

Identifiers

PMID40140541
PMCPMC11947096

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.