Evidence map›Paper›PMID 40140433›Full record

ArticleScientific reports2025

Designing a multi-epitope vaccine against Pseudomonas aeruginosa via integrating reverse vaccinology with immunoinformatics approaches.

Fei Zhu, Rongliu Qin, Shiyang Ma, Ziyou Zhou, Caixia Tan, Hang Yang, Peipei Zhang, Yizhong Xu, Yuying Luo, Jie Chen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
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  6. Review
  7. Article
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  9. Review
  10. Article
  11. Molecular detection ofFrontiers in microbiology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fei Zhu *Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Rongliu Qin *Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Shiyang MaDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Ziyou ZhouDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Caixia TanNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, Hunan, China.
Hang YangDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Peipei ZhangDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yizhong XuDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yuying LuoDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jie ChenDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China. chenjie869@csu.edu.cn.
Pinhua PanDepartment of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China. pinhuapan668@csu.edu.cn.

Funding

the China Postdoctoral Science Foundation 2022M713520the Key R&D Program of Hunan Province No. 2022SK2038the national key clinical specialist construction programs of China Grant Number z047-02the National Natural Science Foundation of China No. 81770080the Natural Science Foundation of Changsha No.kq2208368the Natural Science Foundation of Hunan Province of China No.2023JJ30930the Project Program of central south university graduate education teaching reform No. 2022JGB025the Research Project of Teaching Reform in Colleges and Universities in Hunan Province 2021jy139-2the Research Project on teaching reform of ordinary colleges and universities in Hunan province 2022JGYB037the Scientific Research Program of FuRong Laboratory No.2023SK2101
6 · The paper itself

Abstract

Pseudomonas aeruginosa is a typically opportunistic pathogen responsible for a wide range of nosocomial infections. In this study, we designed two multi-epitope vaccines targeting P. aeruginosa proteins, incorporating cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL), and linear B lymphocyte (LBL) epitopes identified using reverse vaccinology and immunoinformatics approaches. The vaccines exhibited favorable physicochemical properties, including stability, solubility, and optimal molecular weight, suggesting their potential as viable candidates for vaccine development. Molecular docking studies revealed strong binding affinity to Toll-like receptors 1 (TLR1) and 2 (TLR2). Furthermore, molecular dynamics simulations confirmed the stability of the vaccine-TLR complexes over time. Immune simulation analyses indicated that the vaccines could induce robust humoral and cellular immune responses, providing a promising new approach for combating P. aeruginosa infections, particularly in the face of increasing antibiotic resistance.

Indexed as

Epitopes, T-LymphocytePseudomonas aeruginosaPseudomonas InfectionsPseudomonas VaccinesVaccinologyComputational BiologyEpitopes, B-LymphocyteHumansImmunoinformaticsMolecular Docking SimulationMolecular Dynamics SimulationT-Lymphocytes, CytotoxicEpitopes, B-LymphocyteEpitopes, T-LymphocytePseudomonas VaccinesImmunoinformaticsMolecular dockingMolecular dynamics simulationMulti-epitope vaccinePseudomonas aeruginosaReverse vaccinology

Identifiers

PMID40140433
PMCPMC11947098

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.