ArticleEuropean journal of trauma and emergency surgery : official publication of the European Trauma Society2025
Influence of hemolysis, lipemia and bilirubin on biobank sample quality- origin and interference in the use for extracellular vesicle (EV) and MiRNA analyses.
Article in European journal of trauma and emergency surgery : official publication of the European Trauma Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Review
- Extension of the fluidics NTF-Biobank of polytraumatized patients with extracellular vesicles: improved vesicle preservation through local processing.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2026Article
- Quality Evaluation Considerations for Stem Cell-Derived Extracellular Vesicles-Based Therapeutic Products in China.Journal of extracellular vesicles · 2026Review
- Diagnostic and Prognostic Utility of Cell-Surface Vimentin Positive Circulating Tumor Cells in Breast Cancer Using an Automated Negative Selection Platform.Diseases (Basel, Switzerland) · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePre-analytic interferences can influence the laboratory downstream measurements. We recognized hemolysis, lipemia and bilirubin in some of the serum/plasma samples of the NTF-Biobank from polytraumatized patients. Aim of the present study was to detect interferences, find reasons and describe the influence on downstream analyses.
methodsThe study included serum samples of n = 88 polytraumatized patients admitted to a Level 1 Trauma Center in Germany at the ER & up to 10 days after trauma. Optical absorption spectra of UV-VIS (350-660 nm) were measured to detect hemolysis, lipemia and bilirubin. To find reasons for the interferences, clinical parameters like triglycerides (TAGs), nutrition, anaesthesia or transfusions were collected from patients' record. Extracellular vesicles (EVs) were isolated by SEC from controls, lipidemic and hemolytic samples and analysed via NTA.
resultsWithin 10 days after trauma 31.8% of polytraumatized patients' samples showed hemolysis, 12.5% showed increased bilirubin and 15.9% lipemia. Hemolysis occurred in samples mostly at the ER (18%) and was not associated with the number of red blood cell transfusions or the ISS. Both contaminants, hemolysis and lipemia interfered with EV/EV-miRNA measurements. EV miR-16-5p was significantly increased in patients with hemolysis. The presence of lipids further influenced the EV particle size distribution and concentration.
conclusionThe optical absorption spectra measurement is an easy tool for a robust pre-analytic sample controlling for the presence of interferences. Nutrition and anaesthesia were found to be related with lipemia in samples. Hemolysis and lipemia interfered with EV/EV-miRNA analysis. Therefore, the optical absorption spectra pre-analyses should be incorporated in the EV-biobank sampling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.