Evidence map›Paper›PMID 40137877›Full record

ArticleToxins2025

Acyclic Cucurbit[n]uril-Enabled Detection of Aflatoxin B1 via Host-Guest Chemistry and Bioluminescent Immunoassay.

Shaowen Wu, Ke Feng, Jinlu Niu, Jintao Xu, Hualian Mo, Xiaoman She, Shang-Bo Yu, Zhan-Ting Li, Shijuan Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shaowen WuState Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.ORCID 0000-0002-9885-5642
Ke FengState Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China.ORCID 0000-0003-2024-6393
Jinlu NiuState Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Jintao XuState Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Hualian MoState Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Xiaoman SheGuangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Shang-Bo YuState Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China.ORCID 0000-0001-7186-367X
Zhan-Ting LiState Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China.ORCID 0000-0003-3954-0015
Shijuan YanState Key Laboratory of Swine and Poultry Breeding Industry, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.

Funding

Natural Science Foundation of China 22201293Natural Science Foundation of China 22307022Natural Science Foundation of Guangdong Province 2024A1515010062Project of Collaborative Innovation Center of GDAAS XT202210Project of Collaborative Innovation Center of GDAAS XT202504Shanghai Sailing Program 22YF1458300Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science R2023PY-JX024; R2021YJ-YB1004; R2023PY-JG025; 2023QZ-NK04; GDNKY-ZQQZ-K5
6 · The paper itself

Abstract

Aflatoxin B1 (AFB1), a highly toxic secondary metabolite produced by Aspergillus species, represents a significant health hazard due to its widespread contamination of agricultural products. The urgent need for sensitive and sustainable detection methods has driven the development of diverse analytical approaches, most of which heavily rely on organic solvents, posing environmental challenges for routine food safety analysis. Here, we introduce a supramolecular platform leveraging acyclic cucurbit[n]uril (acCB) as a host molecule for environmentally sustainable AFB1 detection. Screening various acCB derivatives identified acCB6 as a superior host capable of forming a stable 1:1 complex with AFB1 in an aqueous solution, exhibiting a high binding affinity. Proton nuclear magnetic resonance (1H NMR) spectroscopy confirmed that AFB1 was deeply encapsulated within the host cavity, with isothermal titration calorimetry (ITC) experiments and molecular dynamics simulations further substantiating the stability of the interaction, driven by enthalpic and entropic contributions. This supramolecular host was incorporated into a scaffold-assembly-based bioluminescent enzyme immunoassay (SA-BLEIA), providing a green detection platform that rivals the performance of traditional organic solvent-based assays. Our findings highlight the potential of supramolecular chemistry as a foundation for eco-friendly mycotoxin detection and offer valuable insights into designing environmentally sustainable analytical methods.

Indexed as

Aflatoxin B1Bridged-Ring CompoundsImidazolesCucurbit(n)urilsFood ContaminationHeterocyclic Compounds, 2-RingImidazolidinesImmunoassayLuminescent MeasurementsMacrocyclic CompoundsAflatoxin B1Bridged-Ring CompoundsCucurbit(n)urilsHeterocyclic Compounds, 2-RingImidazolesImidazolidinesMacrocyclic Compoundsacyclic cucurbit[n]urilsaflatoxin B1bioluminescent immunoassayhost–guest chemistrysupramolecular recognition

Identifiers

PMID40137877
PMCPMC11945384

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.