Evidence map›Paper›PMID 40137574›Full record

ArticleTomography (Ann Arbor, Mich.)2025

A Non-Invasive, Label-Free Method for Examining Tardigrade Anatomy Using Holotomography.

Minh-Triet Hong, Giyoung Lee, Young-Tae Chang

Abstract read
In one paragraph

Article in Tomography (Ann Arbor, Mich.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Minh-Triet HongDepartment of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.ORCID 0000-0002-9426-6832
Giyoung LeeMolecular Imaging Center, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Young-Tae ChangDepartment of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.ORCID 0000-0002-1927-3688

Funding

Korea government (MSIT); Glocal University 30 project 2023R1A2C300453411; RS-2024-00411069
6 · The paper itself

Abstract

BACKGROUND/

objectivesHolotomography is an advanced imaging technique that enables high-resolution, three-dimensional visualization of microscopic specimens without the need for fixation or staining. Here we aim to apply holotomography technology to image live

methodsWe use a low concentration of 7% ethanol for tardigrade sedation and sample preparation. Holotomographic images were obtained and reconstructed using the Tomocube HT-X1 system, enabling high-resolution visualization of tardigrade anatomical structures.

resultsWe captured detailed, label-free holotomography images of both external and internal structures of tardigrade, including the digestive tract, brain, ovary, claws, salivary glands, and musculature.

conclusionsOur findings highlight holotomography as a complementary high-resolution imaging modality that effectively addresses the challenges faced with traditional imaging techniques in tardigrade research.

Indexed as

HolographyImaging, Three-DimensionalTardigradaAnimalsFemaleholotomographylabel-free imagingtardigrade anatomy

Identifiers

PMID40137574
PMCPMC11946113

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.