Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in

Haikun Qi, Haihua Ruan, Tao Wu, Hongyang Zhang, Rui Dong, Yanjun Jiang

Abstract read

In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it

0cells of the map it votes in

4citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

Process optimization for protoplast preparation from Grifola frondosa and development of a high-yield mutant with enhanced mycelial growth and protein production.BMC biotechnology · 2026
Article
An Efficient and Stable PEG-Mediated Transformation System for Medicinal FungusJournal of fungi (Basel, Switzerland) · 2026
Article
Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell CPlant methods · 2026
Article
Establishment and comparative analysis ofFrontiers in plant science · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Haikun QiTianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Haihua RuanTianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Tao WuTianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Hongyang ZhangTianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Rui DongTianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

Yanjun JiangSchool of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.

Funding

China light industry biotransformation and natural product development key laboratory opening-up foundation 2024ZQSWKF004National Natural Science Foundation of China 31870122the Natural Science Foundation of Tianjin 18JCYBJC96000the Youth Program of National Natural Science Foundation of China 21908168

6 · The paper itself

Abstract

PubMed holds no abstract for this paper.

Indexed as

Cordyceps cicadaegenetic transformationoptimizationprotoplasts

Identifiers

PMID40137257

DOI10.3390/jof11030219

PMCPMC11943176

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.