Evidence map›Paper›PMID 40136645›Full record

ArticleCells2025

The Effects of Electrical Stimulation on a 3D Osteoblast Cell Model.

Crystal O Mahadeo, Alireza Shahin-Shamsabadi, Maedeh Khodamoradi, Margaret Fahnestock, Ponnambalam Ravi Selvaganapathy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Crystal O MahadeoNeuroscience Graduate Program, McMaster University, Hamilton, ON L8S 4K1, Canada.ORCID 0000-0002-9303-9830
Alireza Shahin-ShamsabadiSchool of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0002-8145-2556
Maedeh KhodamoradiSchool of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0002-1558-5078
Margaret FahnestockDepartment of Psychiatry and Behavioural Neurosciences, McMaster University, Hamilton, ON L8S 4K1, Canada.ORCID 0000-0002-6815-0529
Ponnambalam Ravi SelvaganapathySchool of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L8, Canada.ORCID 0000-0003-2041-7180

Funding

McMaster University MIRA Catalyst grant
6 · The paper itself

Abstract

Electrical stimulation has been used with tissue engineering-based models to develop three-dimensional (3D), dynamic, research models that are more physiologically relevant than static two-dimensional (2D) cultures. For bone tissue, the effect of electrical stimulation has focused on promoting healing and regeneration of tissue to prevent bone loss. However, electrical stimulation can also potentially affect mature bone parenchymal cells such as osteoblasts to guide bone formation and the secretion of paracrine or endocrine factors. Due to a lack of physiologically relevant models, these phenomena have not been studied in detail. In vitro electrical stimulation models can be useful for gaining an understanding of bone physiology and its effects on paracrine tissues under different physiological and pathological conditions. Here, we use a 3D, dynamic, in vitro model of bone to study the effects of electrical stimulation conditions on protein and gene expression of SaOS-2 human osteosarcoma osteoblast-like cells. We show that different stimulation regimens, including different frequencies, exposure times, and stimulation patterns, can have different effects on the expression and secretion of the osteoblastic markers alkaline phosphatase and osteocalcin. These results reveal that electrical stimulation can potentially be used to guide osteoblast gene and protein expression.

Indexed as

Cell Culture Techniques, Three DimensionalElectric StimulationModels, BiologicalOsteoblastsAlkaline PhosphataseCell Line, TumorHumansOsteocalcinOsteogenesisAlkaline PhosphataseOsteocalcin3D bone construct3D in vitro modelalkaline phosphatasebone tissuecalciumelectrical stimulationhuman osteosarcoma cellshydroxyapatiteosteoblastsosteocalcinSaOS-2 cells

Identifiers

PMID40136645
PMCPMC11941504

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.