Evidence map›Paper›PMID 40136523›Full record

ArticleBiology2025

Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions' Composition During Progressive Passages of Parental Cells.

Elena Donetti, Paola Bendinelli, Margherita Correnti, Elena Gammella, Stefania Recalcati, Anita Ferraretto

Abstract read
In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Mulberroside A attenuates HMolecular medicine reports · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elena DonettiDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0002-5634-5863
Paola BendinelliDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0002-2047-7215
Margherita CorrentiDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0002-1187-3988
Elena GammellaDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0001-9921-8664
Stefania RecalcatiDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0002-2115-8283
Anita FerrarettoDepartment of Biomedical Sciences for Health, University of Milan, 20133 Milan, Italy.ORCID 0000-0002-3637-5002

Funding

University of Milan Piano di sostegno alla ricerca 2023 - Linea 2
6 · The paper itself

Abstract

Epithelial linings are crucial for the maintenance of physiological barriers. The intestinal epithelial barrier (IEB) consists of enterocytes through tight junctions and mucus-secreting cells and can undergo physiological modifications throughout life. To reproduce as closely as possible the IEB main features over time, in vitro co-cultures of Caco2/HT-29 70/30 formed by parental Caco2 and HT-29 cells sub-cultivated for more than 40 passages were set up. The measurements of the transepithelial electrical resistance (TEER) identified two populations: physiological TEER co-cultures (PC) with values > 50 Ωcm

Indexed as

claudin 2co-cultureimmunofluorescenceintestinal epithelial barrierMUC-2occludintransepithelial electrical resistancetransmission electron microscopywestern blot

Identifiers

PMID40136523
PMCPMC11939685

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.