Evidence map›Paper›PMID 40135553›Full record

ArticleBioTechniques2025

Developing an insert to protect large DNA molecules during cell lysis.

Charles Polen, Esmeralda Mendez Ortiz, Cordelia Harbison, Nate Garringer, Martin Lopez, Kristy L Kounovsky-Shafer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Charles PolenDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.
Esmeralda Mendez OrtizDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.
Cordelia HarbisonDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.
Nate GarringerDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.
Martin LopezDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.
Kristy L Kounovsky-ShaferDepartment of Chemistry, University of Nebraska, Kearney, Kearney, NE, USA.ORCID 0000-0002-0544-962X

Funding

UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI PAUL L SORGEN · 2012 to 2026
$59.2M
Development of a system to protect and concentrate large DNA for genome analysisR15GM155799 · NIGMS · UNIVERSITY OF NEBRASKA KEARNEY · PI KOUNOVSKY-SHAFER, KRISTY · 2024 to 2024
$395k
NIGMS NIH HHS P20 GM103427NIGMS NIH HHS R15 GM155799
6 · The paper itself

Abstract

Determining large structural variations is difficult and time-consuming without long molecules to aid in the genome assembly. One issue is the fragility of large DNA molecules during routine molecular biology techniques. A novel inverted agarose insert was created to protect and make accessing large DNA easier. The inverted agarose insert has the cell solution in the middle and the agarose on the outside rather than the cells embedded in the agarose (plug inserts). Multiple agarose concentrations were tested to determine the best percentage of agarose to create the inverted insert. A proof-of-principle inverted insert was tested with

Indexed as

DNADNA, FungalSaccharomyces cerevisiaeSepharoseDNADNA, FungalSepharose3D-printingAgarose insertDNA storageinverted insertplug inserts

Identifiers

PMID40135553
PMCPMC12101932

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.