ArticleBMC complementary medicine and therapies2025
Optimization of Phellinus hartigii extracts: Biological activities, and phenolic content analysis.
Article in BMC complementary medicine and therapies, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Bioactivity assessment and LC-MS/MS-based phenolic characterization of Gypsophila pilosa.Scientific reports · 2026Article
- Artificial intelligence-assisted optimization of Lentinula edodes extracts for enhanced bioactive profile and therapeutic potential.Scientific reports · 2026Article
- Evaluation of phenolic profile and multi-biological activities of Lepista glaucocana extracts optimized by ANN-GA and RSM models.Scientific reports · 2026Article
- Evaluation of the phenolic profile and biological activities of Boletus speciosus extracts optimized with response surface methodology and artificial neural networks-genetic algorithm.Scientific reports · 2026Article
- Phenolic content and biological activities of Lenzites betulina extracts obtained by ultrasonic-assisted optimization approaches.Scientific reports · 2026Article
- Optimization of Hymenopellis radicata extracts using RSM and ANN-GA and evaluation of biological activities.Scientific reports · 2026Article
- Butenolides and triterpenoids from the fungusFrontiers in fungal biology · 2026Article
- RSM and ANN-GA assisted optimization of Morchella importuna extracts and their effects on antioxidant anticholinesterase and antiproliferative activity.Scientific reports · 2025Article
- Maximization of bioactive potential of Fomes fomentarius via artificial Intelligence-Assisted optimization.Scientific reports · 2025Article
- Evaluation of antioxidant, anticholinesterase and antiproliferative potential of Artemisia herba-alba by artificial intelligence-assisted extraction optimization.Scientific reports · 2025Article
- Artificial intelligence-assisted optimization of Eichhornia crassipes extracts and evaluation of their biological activities.Scientific reports · 2025Article
- Geographical variation in antioxidant, anticancer and anticholinesterase activities and phenolic contents of Visnaga daucoides.Scientific reports · 2025Article
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6 authors.
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Abstract
backgroundMedicinal mushrooms are sources of natural substances with diverse biological functions. The study evaluated the biological activity of Phellinus hartigii (Allesch. & Schnabl) Pat. and optimized extraction conditions to the maximize its bioactive potential.
methodsExtraction was performed using a Soxhlet apparatus under varying conditions: temperatures (30, 50, and 70 °C), durations (1, 5.5, and 10 h), and ethanol/water ratios (0%, 50%, and 100%). Total antioxidant status (TAS) was analyzed across 17 experiments, and the optimal conditions were identified using response surface methodology (RSM). Extracts from optimal conditions were further analyzed for antioxidant capacity (Rel assay kits, DPPH, FRAP), anticholinesterase activity (acetyl- and butyrylcholinesterase inhibition), antiproliferative activity (A549 lung cancer cell line), total phenolic content (Folin-Ciocalteu method), and phenolic compound profile (LC-MS/MS).
resultsOptimal extraction conditions were determined to be 48.22 ˚C, 9.04 h, and an ethanol/water ratio of 52.22%. The extract exhibited significant antiproliferative effects against the A549 lung cancer cells, with activity increasing in a concentration-dependent manner. The inhibition values (IC
conclusionsThe extract of P. hartigii obtained under optimal conditions demonstrated substantial antioxidant, anticholinesterase, and antiproliferative activities, highlighting its therapeutic potential.
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