ArticleSe pu = Chinese journal of chromatography2025
[Determination of four oxidative stress biomarkers in human urine using solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry].
Article in Se pu = Chinese journal of chromatography, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Ultraviolet Absorbers Exhibit Dual Modulatory Effects on Oxidative Stress Biomarkers in Exposed Populations.Environment & health (Washington, D.C.) · 2026Article
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Authors and funding
7 authors.
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Abstract
Oxidative stress biomarkers are measurable biological indicators that reflect the balance between the production of reactive oxygen species (ROS) and the body's ability to neutralize them using antioxidants. Elevated oxidative stress is associated with a number of health effects. Herein, we report the development of a comprehensive and sensitive method for quantifying four typical oxidative stress biomarkers in human urine using solid-phase extraction (SPE) in conjunction with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The quantified biomarkers include L,L-dityrosine (diY), 8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), and 4-hydroxynonenal mercapturic acid (HNEMA), which are markers of oxidative-stress-related damage in proteins, DNA, RNA, and lipids, respectively. To that end, we systematically optimized the MS parameters, SPE cartridge, and elution conditions of the method. Briefly, 0.2 mL of a urine sample was mixed with 0.8 mL of pure water, after which an internal-standard mixture was added. The four target analytes were enriched and purified using an HLB SPE cartridge. The diY and the other three compounds were eluted with 2% (volume fraction) methanol aqueous solution and methanol, respectively. The two groups of eluates containing different target analytes were separately injected onto an Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) and gradient eluted using 0.05% (v/v) acetic acid aqueous solution and methanol. The target analytes were identified using both negative and positive electrospray ionization (ESI
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