Evidence map›Paper›PMID 40132593›Full record

ArticleImmunity2025

Long-lasting B cell convergence to distinct broadly reactive epitopes following vaccination with chimeric influenza virus hemagglutinins.

Jenna J Guthmiller, Linda Yu-Ling Lan, Lei Li, Yanbin Fu, Sean A Nelson, Carole Henry, Christopher T Stamper, Henry A Utset, Alec W Freyn, Julianna Han and 27 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Immunity, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

37 authors.

Jenna J GuthmillerDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA; Department on Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address: jenna.guthmiller@cuanschutz.edu.
Linda Yu-Ling LanCommittee on Immunology, University of Chicago, Chicago, IL 60637, USA.
Lei LiDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA; Drukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Yanbin FuDrukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Sean A NelsonDrukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Carole HenryDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Christopher T StamperCommittee on Immunology, University of Chicago, Chicago, IL 60637, USA.
Henry A UtsetDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Alec W FreynDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Julianna HanDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Olivia StovicekDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Jiaolong WangDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Nai-Ying ZhengDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA; Drukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Min HuangDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA; Drukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Haley L DuganCommittee on Immunology, University of Chicago, Chicago, IL 60637, USA.
Micah E TeporaDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Xueyong ZhuDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Yao-Qing ChenDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Anna-Karin E PalmDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA.
Dustin G ShawCommittee on Immunology, University of Chicago, Chicago, IL 60637, USA.
Madhumathi LoganathanDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Benjamin F FrancisDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Jiayi SunDrukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Jordan ChervinDrukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Chloe TroxellDrukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA.
Philip MeadeDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Nancy H L LeungWHO Collaborating Center for Infectious Disease Epidemiology and Control, School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China; Laboratory of Data Discovery for Health Limited, Hong Kong Science and Technology Park, Hong Kong, China.
Sophie A ValkenburgDepartment of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China.
Sarah CobeyDepartment of Ecology and Evolution, University of Chicago, Chicago, IL 60637, USA.
Benjamin J CowlingWHO Collaborating Center for Infectious Disease Epidemiology and Control, School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Ian A WilsonDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA; The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Adolfo García-SastreDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; The Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Raffael NachbagauerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Andrew B WardDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Lynda CoughlanDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Center for Vaccine Development and Global Health (CVD), University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Florian KrammerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Patrick C WilsonDepartment of Medicine, Section of Rheumatology, University of Chicago, Chicago, IL 60637, USA; Committee on Immunology, University of Chicago, Chicago, IL 60637, USA; Drukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USA. Electronic address: pcw4001@med.cornell.edu.

Funding

COLLABORATIVE INFLUENZA VACCINE INNOVATION CENTER: UNIVERSAL INFLUENZA VACCINE RESEARCH75N93019C00051 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KRAMMER, FLORIAN · 2019 to 2025
$105.4M
VACCINE INDUCED IMMUNITY IN THE YOUNG AND AGEDU19AI057266 · NIAID · EMORY UNIVERSITY · PI Rafi Ahmed · 2003 to 2026
$81.7M
NIAID Centers of Excellence for Influenza Research and Response: Universal Influenza Vaccine Research Activities75N93021C00014 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GARCIA-SASTRE, ADOLFO · 2021 to 2025
$62.6M
MEDICAL SCIENTIST TRAININGT32GM007281 · NIGMS · UNIVERSITY OF CHICAGO · PI CLARK, MARCUS RAMSAY · 1985 to 2022
$32.1M
Toward a Universal Influenza Virus VaccineP01AI097092 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI PALESE, PETER · 2012 to 2022
$17.6M
Viral Immunity and VAccination (VIVA) Human Immunology Project Consortium (HIPC)U19AI168631 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Ana Fernandez-Sesma · 2022 to 2026
$14.4M
Understanding Human B Cell Responses to Influenza to Improve the Influenza U19AI109946 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI PALESE, PETER · 2014 to 2018
$9.6M
PATHOGENESIS AND IMMUNOLOGY OF DISEASET32AI007244 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI NEMAZEE, DAVID · 1985 to 2022
$8.8M
University of Chicago Autoimmunity Center of ExcellenceU19AI082724 · NIAID · UNIVERSITY OF CHICAGO · PI WILSON, PATRICK CHRISTOPHER · 2009 to 2023
$7.2M
The "Dynamics of the immune responses to repeat influenza vaccination exposures" (DRIVE) StudyU01AI153700 · NIAID · UNIVERSITY OF CHICAGO · PI COBEY, SARAH, COWLING, BENJAMIN JOHN · 2020 to 2024
$6.2M
Medical Scientist National Research Service AwardT32GM150375 · NIGMS · UNIVERSITY OF CHICAGO · PI Raghavendra G Mirmira · 2023 to 2026
$5.5M
Generation of a polycistronic universal influenza virus vaccine based on rare species adenoviral vectorsR21AI146529 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI COUGHLAN, LYNDA · 2019 to 2020
$466k
NIAID NIH HHS 75N93019C00051NIAID NIH HHS 75N93021C00014NIAID NIH HHS K99 AI159136NIAID NIH HHS P01 AI097092NIAID NIH HHS R00 AI159136NIAID NIH HHS R21 AI146529NIAID NIH HHS T32 AI007244NIAID NIH HHS U01 AI153700NIAID NIH HHS U19 AI057266NIAID NIH HHS U19 AI082724NIAID NIH HHS U19 AI109946NIAID NIH HHS U19 AI168631NIGMS NIH HHS T32 GM007281NIGMS NIH HHS T32 GM150375NIH HHS HHSN272201400005CNIH HHS HHSN272201400008C
6 · The paper itself

Abstract

In a phase 1 clinical trial, a chimeric hemagglutinin (cHA) immunogen induced antibody responses against the conserved hemagglutinin (HA) stalk domain as designed. Here, we determined the specificity, function, and subsets of B cells induced by cHA vaccination by pairing single-cell RNA sequencing and B cell receptor repertoire sequencing. We have shown that the cHA-inactivated vaccine with a squalene-based adjuvant induced a robust activated B cell and memory B cell (MBC) phenotype against two broadly neutralizing epitopes in the stalk domain. The overall specificities of the acute plasmablast (PB) and MBC responses clonally overlapped, suggesting B cell convergence to these broadly protective epitopes. At 1 year post immunization, we identified that cHA vaccination reshaped the HA-specific MBC pool to enrich for stalk-binding B cells. Altogether, these data indicate the cHA vaccine induced robust and durable B cell responses against broadly protective epitopes of the HA stalk domain, in line with serological data.

Indexed as

B-LymphocytesEpitopes, B-LymphocyteHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesMemory B CellsAntibodies, NeutralizingAntibodies, ViralHumansImmunologic MemoryInfluenza, HumanReceptors, Antigen, B-CellVaccinationAntibodies, NeutralizingAntibodies, ViralEpitopes, B-LymphocyteHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesReceptors, Antigen, B-Cellbroadly neutralizing epitopeschimeric hemagglutinindurable B cell responsesHA anchor epitopeHA stalk epitopeinfluenzanext-generation influenza vaccineuniversal influenza vaccine

Identifiers

PMID40132593
PMCPMC11981830

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.