Protocol for evaluating neuronal activity and neurotransmitter release following amyloid-beta oligomer injections into the rat hippocampus.

Vincent Hervé, Laurie Bonenfant, Mathilde Amyot, Rime Balafrej, Obai Bin Ka'B Ali, Habib Benali, Jonathan Brouillette

Abstract read

In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Vincent HervéDepartment of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada. Electronic address: vincent.herve@umontreal.ca.

Laurie BonenfantDepartment of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada.

Mathilde AmyotDepartment of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada.

Rime BalafrejDepartment of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada.

Obai Bin Ka'B AliDepartment of Electrical and Computer Engineering, Concordia University, Montréal, QC, Canada.

Habib BenaliDepartment of Electrical and Computer Engineering, Concordia University, Montréal, QC, Canada.

Jonathan BrouilletteDepartment of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada. Electronic address: jonathan.brouillette@umontreal.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In Alzheimer's disease, there is an imbalance in neurotransmitter release and altered neuronal activation. Here, we present a protocol approach to analyze neuronal activity by combining local field potential (LFP) recording with microdialysis within the same animal. We describe steps for measuring glutamate and GABA levels following hippocampal amyloid-beta oligomer (Aβo) injections in rats. We then detail procedures for assembling the electrode and cannula, surgical implantation and simultaneous in vivo LFP recording, interstitial fluid collection, and Aβo injections.

Indexed as

Amyloid beta-PeptidesHippocampusNeuronsNeurotransmitter AgentsAnimalsgamma-Aminobutyric AcidGlutamic AcidMaleMicrodialysisRatsAmyloid beta-Peptidesgamma-Aminobutyric AcidGlutamic AcidNeurotransmitter AgentsMass spectrometryNeuroscienceSystems biology

Identifiers

PMID40131934

DOI10.1016/j.xpro.2025.103712

PMCPMC12224876

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.