Evidence map›Paper›PMID 40131505›Full record

ArticleMedical microbiology and immunology2025

CRISPR/Cas9-mediated deletion of a kinetoplast-associated gene attenuates virulence in Leishmania major parasites.

Fatemeh Darzi, Ali Khamesipour, Minoo Tasbihi, Maryam Bahraminasab, Mahmoud Nateghi-Rostami

Abstract read
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In one paragraph

Article in Medical microbiology and immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Fatemeh DarziDepartment of Parasitology, Pasteur Institute of Iran, 12 Farvardin St, Tehran, 1316943551, Iran.
Ali KhamesipourCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
Minoo TasbihiCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
Maryam BahraminasabDepartment of Biotechnology, Faculty of Sciences, University of Tehran, Tehran, Iran.
Mahmoud Nateghi-RostamiDepartment of Parasitology, Pasteur Institute of Iran, 12 Farvardin St, Tehran, 1316943551, Iran. m_nateghi@pasteur.ac.ir.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We employed a CRISPR/Cas9 technique in Leishmania major to evaluate its efficiency in editing a kDNA-associated gene, the universal minicircle sequence binding protein (UMSBP), which is involved in mitochondrial respiration and kinetoplast division. Using this toolkit, we generated UMSBP mNG-tagged and single knockout L. major (LmUMSBP

Indexed as

CRISPR-Cas SystemsDNA, KinetoplastGene DeletionLeishmania majorLeishmaniasis, CutaneousProtozoan ProteinsAnimalsCytokinesDisease Models, AnimalFemaleGene EditingMacrophagesMiceMice, Inbred BALB CVirulenceCytokinesDNA, KinetoplastProtozoan ProteinsCRISPR/Cas9Genome editingKinetoplastLeishmania majorLive attenuated vaccinesTh1 immune response

Identifiers

PMID40131505

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.