ArticleCureus2025
Impact of Demineralization Time on Enamel Microhardness Reduction and Lesion Depth: An In Vitro Study.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Biomimetic Strategies for Subsurface Enamel Remineralization: Depth-Dependent Effects of Biomimetic Agents Compared with Fluoride.Biomimetics (Basel, Switzerland) · 2026Article
- In vitro assessment of experimental bioactive tooth gel formulas containing L-arginine, ginger and eggshell for remineralization and anti-bacterial effect.Scientific reports · 2026Article
- Influence of White Spot Lesion Severity on the Structural, Optical, and Mechanical Outcomes of Resin Infiltration: An In Vitro Study.Diagnostics (Basel, Switzerland) · 2026Article
- Assessment of toothbrushing, bleaching pen and bleaching mouthwash in removing stains from tooth structure and single-shade resin composite.Scientific reports · 2026Article
- Comparative evaluation of remineralisation potential using BioHAP, CPP-ACP and Enamelon on root dentin: AnBioinformation · 2026Article
- TheBiomaterial investigations in dentistry · 2026Article
- Salivary proteins, proteases, and disease pathophysiology in oral health and precision diagnostics: a narrative review.Frontiers in dental medicine · 2026Review
- Relationship of finger dermatoglyphics with ameloglyphics and their values as dental caries predictors in primary teeth.BMC oral health · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe duration of acid exposure is a critical factor in determining an individual's risk of developing caries. The objective of this study was to investigate the correlation between demineralization time and two key parameters: microhardness and depth of an enamel caries lesion.
methodsSixty recently extracted human teeth were mounted in acrylic resin and randomly divided into five groups (n = 12). First, 400 grit and then 1,000 grit sandpaper were applied to the buccal surfaces for five minutes each. Other surfaces, except buccal surfaces, were coated with an acid-resistant varnish. The groups were stored in the same demineralization solution (pH 4.35-4.65), respectively, for a duration of 60, 72, 84, 96, and 108 hours. Before and after exposure to acidic solutions, the surface microhardness of samples (SMH) was evaluated using a Vickers pyramid diamond tip with a 100 gram load applied for 15 seconds. Three measurements were taken from each sample surface, and the average sample value was obtained by averaging the measurements. The depth of the lesions in three specimens from each group was evaluated from five different demineralized pits under a stereomicroscope. Data were analyzed by one-way ANOVA and post-hoc Tukey's honestly significant difference (HSD) tests.
resultsAfter acid exposure, SMH values significantly decreased in all groups (p = 0.001; p < 0.01) Statistically significant differences were shown between all groups in terms of Δhardness (p < 0.05). After 72 hours of demineralization, the microhardness values tend to decrease more as the exposure time increases (p = 0.001; p < 0.01). The mean lesion depths were varied from 4.01 ± 0.71 µm to 13.7 ± 1.17 µm.
conclusionOur findings show that there is a positive correlation between the duration of demineralization and lesion depth and a negative correlation between the duration of demineralization and microhardness. We assume that initial lesions may deepen quickly, especially after 72 hours.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.