Evidence map›Paper›PMID 40129186›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two-Photon Fluorescent Probe.

Hong Huang, Huiru Li, Yong Zhang, Xuhan Xia, Ningwen Zhang, Haixin Fan, Longhua Guo, Yongyong Cao, Hu Pan, Ruijie Deng and 3 more

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hong HuangCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Huiru LiCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Yong ZhangCollege of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, China.
Xuhan XiaCollege of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, China.
Ningwen ZhangCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Haixin FanCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Longhua GuoCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Yongyong CaoCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Hu PanCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Ruijie DengCollege of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, China.ORCID https://orcid.org/0000-0001-9094-124X
Yangang WangCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.
Rodrigo Ledesma-AmaroDepartment of Bioengineering, Imperial College Centre for Synthetic Biology, Imperial College London, London, SW7 2AZ, UK.
Jianguo XuCollege of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China.

Funding

Anhui Provincial Natural Science Foundation 2308085MB57Key Research and Development Program of Anhui Province 2022e07020060National Key R&D Program of China 2022YFF1103000National Natural Science Foundation of China 22074100Special Project for Young Scientific and Technological Talents of Jiaxing City 2023AY40021
6 · The paper itself

Abstract

Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real-time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual-emission two-photon fluorescent probe (named DST) is designed for the simultaneous tracking of tyrosinase (TYR) and adenosine triphosphate (ATP), thereby enabling the real-time monitoring of both neurocellular redox homeostasis and energy metabolism in brain tissue. The developed DST probe exhibits excellent sensitivity and selectivity toward TYR and ATP, with distinctive responses in the blue and red fluorescence channels being observed without spectra crosstalk. Using this probe, the correlation and regulatory mechanism between TYR and ATP during oxidative stress are uncovered. Additionally, the two-photon nature of this probe allows alterations in the TYR and ATP levels to be monitored across different brain regions in an Alzheimer's disease (AD) mouse model. Notably, a significant decrease in ATP levels is revealed within the somatosensory cortex (S1BF) and caudate putamen brain regions of an AD mouse, alongside an increase in TYR levels within the S1BF and laterodorsal thalamic nucleus brain regions. These findings indicate the potential of applying the spatially resolved regulation of neurocellular redox homeostasis and energy metabolism to treat neurodegenerative diseases.

Indexed as

Adenosine TriphosphateBrainFluorescent DyesMonophenol MonooxygenaseAlzheimer DiseaseAnimalsDisease Models, AnimalEnergy MetabolismHumansMiceMice, Inbred C57BLOxidation-ReductionOxidative StressAdenosine TriphosphateFluorescent DyesMonophenol Monooxygenasealzheimer's diseaseATPbrain tissuestwo‐photon imagingtyrosinase

Identifiers

PMID40129186
PMCPMC12097068

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.