Evidence map›Paper›PMID 40128764›Full record

ArticlePlant methods2025

A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny.

Zheng Li, Bingyu Zhang, Yuchen Fu, Yutian Suo, Yinan Zhang, Jinxia Feng, Long Pan, Wanna Shen, Huixiang Liu, Xiaohua Su and 1 more

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In one paragraph

Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zheng Li *State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Bingyu Zhang *State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Yuchen FuState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Yutian SuoState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Yinan ZhangState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Jinxia FengState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Long PanState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Wanna ShenState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China.
Huixiang LiuShandong Research Center for Forestry Harmful Biological Control Engineering and Technology, College of Plant Protection, Shandong Agricultural University, Taian, China.
Xiaohua SuState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Jiaping ZhaoState Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China. zhaojiaping@caf.ac.cn.

Funding

the Central Public-interest Scientific Institution Basal Research Fund of the State Key Laboratory of Tree Genetics and Breeding CAFYBB2020ZY001-2the National Natural Science Foundation of China 32171776
6 · The paper itself

Abstract

backgroundHybrid breeding, a direct and efficient strategy for disease control and management in tree species, is currently limited by the selection method of resist clones: the "in vitro stem segment inoculation method". This method, constrained by the availability of inoculating materials, cannot rapidly, efficiently, and cost-effectively screen the resistance of all hybrid clones. To overcome these limitations, we introduce a novel pathogen inoculation method for the resistance assessment of hybrid clones in the poplar-Valsa sordida pathosystem. This method involves inoculating the stem canker pathogen on the host leaf, a unique and promising approach we have successfully validated.

resultsResults showed that stem canker pathogen V. sordida induced the extended necrotic lesion and even induced the formation of pycnidium structure and conidia on the leaf surface 5 days after mycelium inoculation; (1) the upper 5-7thleaves exhibited higher resistance than the middle 18-20th leaves; (2) the shading conditions induced more severe symptoms on the leaves than lighting conditions; (3) the poplar leaves were more susceptible to the juvenile mycelium inoculums (4-day-cultured) than the old ones (7-day-cultured). Our results demonstrate the robustness ofthe "in vivo leaf inoculation method" in revealing the resistance differentiation in poplar hybrid clones. According to the leaf necrotic area disease index, we divided these poplar clones into seven different resistance groups. The resistance assessed by leaf assessment was validated in 15 selected poplar clones using the "in vitro stem segment inoculation method". Results showed that the effectiveness of these two methods was consistent. Moreover, results also revealed the pathogenicity diversity of the pathogen population of tree species using leaf the inoculation method.

conclusionsCompared to the conventional "in vitro stem segment inoculation method", the leaf method has the advantages of abundant inoculation materials, easy operation, rapid disease onset, and almost no adverse effect on the host. It is particularly suitable for the resistance screening of all progeny and the early (seedling) phenotypic selection of resistant poplar clones in poplar stem disease resistance breeding. The "in vivo leaf inoculation method" holds significant promise in poplar breeding, tree pathology, and molecular biology research on tree stem diseases.

Indexed as

In vivo leaf inoculationPoplar hybrid breedingStem cankerTree diseasesValsa sordida

Identifiers

PMID40128764
PMCPMC11934469

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