Evidence map›Paper›PMID 40128732›Full record

ArticleBMC medical genomics2025

Exploring diagnostic m6A regulators in primary open-angle glaucoma: insight from gene signature and possible mechanisms by which key genes function.

Xinyue Zhang, Jiawei Chen, Xiaoyu Zhou, Dengming Zhou, Li Liao, Yang Zhao, Ping Wu, Fen Nie, Zhimin Liao, Ziyan Cai and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC medical genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinyue Zhang *Hunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Jiawei Chen *Hunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Xiaoyu ZhouHunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Dengming ZhouThe Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Li LiaoAier School of Ophthalmology, Central South University, Changsha, Hunan, China.
Yang ZhaoHunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Ping WuAier School of Ophthalmology, Central South University, Changsha, Hunan, China.
Fen NieHunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Zhimin LiaoHunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China.
Ziyan CaiDepartment of Ophthalmology, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, China.
Xuanchu DuanHunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials, Glaucoma Institute, Changsha Aier Eye Hospital, Changsha, Hunan, China. duanxchu@csu.edu.cn.

Funding

Changsha Municipal Natural Science Foundation kq2208495Hunan Engineering Research Center for Glaucoma with Artificial Intelligence in Diagnosis and Application of New Materials 2023TP2225Natural Science Foundation of Hunan Province, China 2023JJ40003Natural Science Foundation of Hunan Province, China 2023JJ40004Natural Science Foundation of Hunan Province, China 2023JJ70014Science and Technology Foundation of Aier Eye Hospital Group, China AR2206D2Science and Technology Foundation of Aier Eye Hospital Group, China AR2206D4Science and Technology Foundation of Aier Eye Hospital Group, China AR2206D5
6 · The paper itself

Abstract

purposeThe purpose of this study was to interrogate the potential role of N6-methyladenosine (m6A) regulators in the process of trabecular meshwork (TM) tissue damage in patients with primary open-angle glaucoma (POAG).

methodsFirstly, the expression profile of m6A regulators in TM tissues of POAG patients was comprehensively analyzed by bioinformatics analysis; Plasmid transfection and siRNA gene interference were used to enhance or weaken the expression levels of YTHDC2 in human trabecular meshwork cells (HTMCs); Cell migration ability was detected by transwell chamber assay; Immunofluorescence staining assay was used to evaluate the expression of extracellular matrix (ECM) related proteins.

resultsThrough the analysis of GSE27276 database, 5 m6A regulators with different expression in POAG were screened out. The results of random forest model showed that these 5 m6A regulators exhibited diagnostic potential and were characteristic genes of POAG. All POAG samples could be effectively divided into two groups based on the expression levels of these 5 hub m6A regulators. Immune cell infiltration analysis indicated that the levels of activated CD8

conclusionsThe differentially expressed m6A regulators in TM tissues may serve as potential diagnostic biomarkers for POAG. And, in HTMCs, the expression level of YTHDC2 mRNA was changed under oxidative stress or TGF-β2 intervention, and then exerted its regulation on cell migration and ECM synthesis capability through m6A modification, which may be an important part of the disease process of POAG.

Indexed as

AdenosineGlaucoma, Open-AngleTranscriptomeCell MovementGene Expression ProfilingGene Expression RegulationHumansOxidative StressTrabecular MeshworkAdenosineN-methyladenosineGlaucomaN6-methyladenosineOxidative stressPOAGTrabecular meshwork

Identifiers

PMID40128732
PMCPMC11931807

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.