Evidence map›Paper›PMID 40127237›Full record

ArticleACS synthetic biology2025

Ultra-efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids.

Alexander Olek Pisera, Yutong Yu, Rory L Williams, Chang C Liu

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Alexander Olek PiseraDepartment of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Yutong YuDepartment of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Rory L WilliamsDepartment of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Chang C LiuDepartment of Biomedical Engineering, University of California, Irvine, California 92617, United States.ORCID 0000-0002-3290-2880

Funding

Making antibody generation rapid, scalable, and democratic through machine learning and continuous evolutionR01CA260415 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI KRUSE, ANDREW, LIU, CHANG C · 2020 to 2024
$8.4M
Synthetic genetic systems for rapid biomolecular evolution in vivoR35GM136297 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LIU, CHANG C · 2020 to 2024
$2.4M
Visual Sciences Training Program (VSTP)T32EY032448 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Philip David Kiser, Chang C Liu · 2022 to 2026
$656k
NCI NIH HHS R01 CA260415NEI NIH HHS T32 EY032448NIGMS NIH HHS R35 GM136297
6 · The paper itself

Abstract

Efficient methods for diversifying genes of interest (GOIs) are essential in protein engineering. For example, OrthoRep, a yeast-based orthogonal DNA replication system that achieves the rapid

Indexed as

Gene LibraryPlasmidsSaccharomyces cerevisiaeCytosolDNA ReplicationIntegrasesProtein EngineeringSingle-Domain AntibodiesIntegrasesSingle-Domain AntibodiesContinuous EvolutionGene LibrariesIntegraseOrthoRepProtein EngineeringYeast Transformation

Identifiers

PMID40127237
PMCPMC13335410

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.