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ArticleVeterinary research communications2025

Prevalence rates of Babesia caballi and Theileria equi in the horse population of northern Spain: a serological and molecular study.

María Paz Peris, María Serrano, Antonio Romero, Mirta García, Nabil Halaihel, Juan Antonio Castillo, María Jesús Gracia

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Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

María Paz PerisDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain. pazperis@unizar.es.ORCID http://orcid.org/0000-0001-5750-3643
María SerranoDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.
Antonio RomeroDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.
Mirta GarcíaDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.
Nabil HalaihelDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.
Juan Antonio CastilloDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.
María Jesús GraciaDepartment of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Equine piroplasmosis (EP), a tick-borne disease caused by Babesia caballi and Theileria equi, is of significant concern due to its impact on the international horse trade. According to standards established by the World Organisation for Animal Health (WOAH), horses imported from EP-endemic regions must have a certificate confirming negative serological and molecular test results. In EP-free countries, only seronegative horses are allowed to enter. This entails economic losses for endemic regions such as Spain. Our study aimed to assess the prevalence of B. caballi and T. equi in horses from northern Spain and to compare indirect fluorescent antibody tests (IFAT) with polymerase chain reaction (PCR) regarding diagnostic accuracy. In March 2019, blood samples were collected from 50 horses at a slaughterhouse in Zaragoza. The horses originated from meat farms in the Pyrenees and Cantabrian coastal regions, including northern Cantabria, the Basque Country, and Navarre. Serological results revealed a prevalence of 50% for B. caballi, 30% for T. equi, and 16% for mixed infections, with an overall EP prevalence of 64%. PCR results confirmed 22% positive for B. caballi, 42% for T. equi, and 16% for both parasites in one animal, showing an overall EP prevalence of 54%. Our study underscores the critical need for combining serological and molecular diagnostic tests to accurately identify carriers and active infections, with the goal of ensuring safe animal movement and controlling EP transmission.

Indexed as

BabesiaBabesiosisHorse DiseasesTheileriaTheileriasisAnimalsFemaleFluorescent Antibody Technique, IndirectHorsesMalePolymerase Chain ReactionPrevalenceSeroepidemiologic StudiesSpainEquine piroplasmosisNorthern SpainReal-time PCRSeroprevalence

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.