Evidence map›Paper›PMID 40124668›Full record

ArticleBiology methods & protocols2025

A protocol for karyotyping and genetic editing of induced pluripotent stem cells with homology-directed repair mediated CRISPR/Cas9.

Silvana Lobo, Rita Barbosa-Matos, Sofia Dória, Ana Maria Pedro, Ana Brito, Daniel Ferreira, Carla Oliveira

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology methods & protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Silvana Loboi3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.ORCID https://orcid.org/0000-0002-5705-2958
Rita Barbosa-Matosi3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.ORCID https://orcid.org/0000-0003-0794-4912
Sofia DóriaGenetics Unit, Department of Pathology, Faculty of Medicine, University of Porto, Porto, 4200-319, Portugal.ORCID https://orcid.org/0000-0001-9225-9076
Ana Maria Pedroi3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.ORCID https://orcid.org/0009-0007-1159-3621
Ana Britoi3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.
Daniel Ferreirai3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.ORCID https://orcid.org/0000-0002-9712-1460
Carla Oliveirai3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, 4200-135, Portugal.ORCID https://orcid.org/0000-0001-8340-2264

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR/Cas9-mediated homology-directed repair (HDR) allows precise gene editing, but its efficiency remains low for certain cell types, such as human induced pluripotent stem cells (hiPSCs). In this study, we aimed to introduce the

Indexed as

CRISPR/Cas9CTNNA1HDRhiPSCskaryotypingprecise genetic editing

Identifiers

PMID40124668
PMCPMC11930342

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.