ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Single-Cell and Spatial Transcriptomics Reveals a Stereoscopic Response of Rice Leaf Cells to Magnaporthe oryzae Infection.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- Molecular Bases and Genetic Design of Rice Disease Resistance for Optimized Yield and Sustainable Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Advances in single-cell and spatial multi-omics for plant development and stress responses.Plant diversity · 2026Review
- Plant Spatio-Temporal Integration Network (PSTN): a framework for dynamic single-cell and spatial transcriptomics in fungal-plant interactions.Bioinformatics (Oxford, England) · 2026Article
- A spatiotemporal atlas of giant-cell formation during Meloidogyne incognita infection in tomato roots.Molecular horticulture · 2026Article
- Single-Cell and Spatial Omics Technologies in Rice Abiotic Stress Biology: A Methodological Review.International journal of molecular sciences · 2026Review
- Single-cell insights into plant growth, adaptation, and evolution.Journal of integrative plant biology · 2026Review
- Metabolomic and Transcriptomic Responses ofPlants (Basel, Switzerland) · 2026Article
- Improved laser capture microdissection combined with transcriptome sequencing reveals gene expression reprogramming during rice panicle branch meristems differentiation.BMC plant biology · 2026Article
- Empowering fungal infection research with single-cell RNA sequencing.Communications biology · 2026Review
- From triangle to pyramid: Understanding host-pathogen-microniome-environment interplay for sustainable, enviromics-empowered management of plant diseases.Plant communications · 2026Review
- A four-dimensional spatial transcriptome atlas of barley caryopsis development and germination.The Plant cell · 2026Article
- The potential of wheat spatial omics.Nature genetics · 2026Review
- Integrated Single-Cell and Spatial Transcriptomics Reveal Cell-Type-Specific Immune Regulatory Networks in Maize Responding to Southern Corn Rust.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Decoding plant physiology through systems biology: Integrative multi-omics and computational perspectives for next-generation crop design.Plant communications · 2026Review
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Integrating Single-Cell and Spatial Multi-Omics to Decode Plant-Microbe Interactions at Cellular Resolution.Microorganisms · 2026Review
- Integrated experimental and computational workflows for single-cell transcriptomics in plants.Plant methods · 2026Article
- Hormone priming and metabolic engineering of phytohormone crosstalk in rice under combined biotic and abiotic stresses: a multi-omics perspective for climate-resilient crop development.Frontiers in plant science · 2026Review
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
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16 authors.
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Abstract
Infection by the fungal pathogen Magnaporthe oryzae elicits dynamic responses in rice. Utilizing an integrated approach of single-cell and spatial transcriptomics, a 3D response is uncovered within rice leaf cells to M. oryzae infection. A comprehensive rice leaf atlas is constructed from 236 708 single-cell transcriptomes, revealing heightened expression of immune receptors, namely Pattern Recognition Receptors (PRRs) and Nucleotide-binding site and leucine-rich repeat (NLRs) proteins, within vascular tissues. Diterpene phytoalexins biosynthesis genes are dramatically upregulated in procambium cells, leading to an accumulation of these phytoalexins within vascular bundles. Consistent with these findings, microscopic observations confirmed that M. oryzae is prone to target leaf veins for invasion, yet is unable to colonize further within vascular tissues. Following fungal infection, basal defenses are extensively activated in rice cells, as inferred from trajectory analyses. The spatial transcriptomics reveals that rice leaf tissues toward leaf tips display stronger immunity. Characterization of the polarity gene OsHKT9 suggests that potassium transport plays a critical role in resisting M. oryzae infection by expression along the longitudinal axis, where the immunity is stronger toward leaf tip. This work uncovers that there is a cell-specific and multi-dimensional (local and longitudinal) immune response to a fungal pathogen infection.
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