ArticleMicroscopy research and technique2025
Characterization of Silver Nanoparticles Synthesized Using Hypericum perforatum L. and Their Effects on Staphylococcus aureus.
Article in Microscopy research and technique, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Biogenic Silver Nanoparticles from the Cell-Free Supernatant of Mychonastes sp. B1: Antibacterial and Antibiofilm Effects, and Wound Healing Activity Supported by Gene and Protein Expression Analysis.Molecular biotechnology · 2026Article
- Targeting Pathogenic Infection and Virulence Genes of Enterococcus Faecalis Using Lactoferrin Bismuth Nano-form.Probiotics and antimicrobial proteins · 2026Article
- One-pot fabrication and characterization of ZnO nanoflowers using Zanthoxylum simulans and evaluating their biomedical claims.Mikrochimica acta · 2026Article
- Effect of Biosynthesized Gold and Silver Nanoparticles UsingAntibiotics (Basel, Switzerland) · 2026Article
- Green synthesis of silver nanoparticles mediated by Solanum nigrum leaf extract and their antifungal activity against pine pathogens.Scientific reports · 2025Article
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Authors and funding
4 authors.
Funding
Abstract
This study investigates the synthesis of silver nanoparticles (AgNPs) using Hypericum perforatum L. and evaluates their antibacterial and antibiofilm activities against Staphylococcus aureus. The synthesized AgNPs were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). UV-Vis spectroscopy showed a maximum absorption peak at 448 nm, which indicates that nanoparticles have been formed successfully. TEM analysis showed that the AgNPs were spherical, with an average size of 35 ± 2.7 nm. FTIR confirmed the presence of functional groups on the surface of AgNP that may be contributing to its biological activity. The AgNPs exhibited significant antibacterial activity, with a minimum inhibitory concentration (MIC) of 75 μg/mL and an inhibition zone of 13 ± 0.13 mm at this concentration. They were also highly effective in inhibiting biofilm formation even at a concentration of 25 μg/mL, reducing biofilm formation by 47.25% ± 3.51%. At increased concentrations, nanoparticles have been shown to compromise bacterial membranes, leading to significant membrane disruption. This disruption subsequently results in a reduction of cellular respiration, with observed decreases of approximately twofold when compared to controls. Additionally, nanoparticles facilitate the production of superoxide anions, which can rise by about threefold, consequently enhancing the overall effectiveness of bacterial inactivation. Field emission scanning electron microscopy (FE-SEM) revealed structural damage to bacterial cells treated with AgNPs, supporting their antimicrobial effects. These findings suggest that AgNPs synthesized from H. perforatum could serve as effective antimicrobial agents against S. aureus. Their ability to disrupt bacterial cell membranes, inhibit respiration, and induce oxidative stress makes them promising candidates for antimicrobial and antibiofilm applications, particularly given the increasing concern over bacterial resistance to conventional antibiotics.
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