Evidence map›Paper›PMID 40121601›Full record

ReviewThe Plant journal : for cell and molecular biology2025

Beyond a few bases: methods for large DNA insertion and gene targeting in plants.

Katie Vollen, Jose M Alonso, Anna N Stepanova

Abstract readReview
In one paragraph

Review in The Plant journal : for cell and molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Plants (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Katie VollenDepartment of Plant Biology, North Carolina State University, Raleigh, North Carolina, 27695, USA.
Jose M AlonsoDepartment of Plant Biology, North Carolina State University, Raleigh, North Carolina, 27695, USA.
Anna N StepanovaDepartment of Plant Biology, North Carolina State University, Raleigh, North Carolina, 27695, USA.ORCID 0000-0003-1018-4758

Funding

National Science Foundation 1444561National Science Foundation 1750006National Science Foundation 1940829National Science Foundation 2023356574National Science Foundation 2327912
6 · The paper itself

Abstract

Genome editing technologies like CRISPR/Cas have greatly accelerated the pace of both fundamental research and translational applications in agriculture. However, many plant biologists are functionally limited to creating small, targeted DNA changes or large, random DNA insertions. The ability to efficiently generate large, yet precise, DNA changes will massively accelerate crop breeding cycles, enabling researchers to more efficiently engineer crops amidst a rapidly changing agricultural landscape. This review provides an overview of existing technologies that allow plant biologists to integrate large DNA sequences within a plant host and some associated technical bottlenecks. Additionally, this review explores a selection of emerging techniques in other host systems to inspire tool development in plants.

Indexed as

DNA, PlantGene EditingGene TargetingMutagenesis, InsertionalPlantsCRISPR-Cas SystemsCrops, AgriculturalGenome, PlantPlant BreedingPlants, Genetically ModifiedDNA, PlantBACgene targetinggenome editinginsertionintegraselarge DNArecombinaserecombinationT‐DNAtransposon

Identifiers

PMID40121601
PMCPMC11930290

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.