Development of an improved construct for spectinomycin selection in plant transformation.

Kent F McCue, Min Shao, Jennie Huynh, Tai Minh, Mandy Chan, York Moy, James G Thomson

Abstract read

In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

A simplePlant biotechnology (Tokyo, Japan) · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kent F McCueUSDA-ARS Crop Improvement and Genetics Research Unity, 800 Buchanan Street, Albany, CA, 94710, USA.

Min ShaoUSDA-ARS Crop Improvement and Genetics Research Unity, 800 Buchanan Street, Albany, CA, 94710, USA.

Jennie HuynhUSDA-ARS Crop Improvement and Genetics Research Unity, 800 Buchanan Street, Albany, CA, 94710, USA.

Tai MinhAlpine Bio, South San Francisco, CA, 94080, USA.

Mandy ChanAlpine Bio, South San Francisco, CA, 94080, USA.

York MoyAlpine Bio, South San Francisco, CA, 94080, USA.

James G ThomsonUSDA-ARS Crop Improvement and Genetics Research Unity, 800 Buchanan Street, Albany, CA, 94710, USA. James.Thomson@usda.gov.

Funding

Agricultural Research Service 2030 21220-003-00D

6 · The paper itself

Abstract

Efficient selection of transgenic plants is essential in plant biotechnology, and development of effective selectable marker systems plays a crucial role, especially as multiple rounds of transformation and gene stacking may require multiple selectable markers. The present study aims to develop and evaluate an enhanced spectinomycin resistance construct to provide robust selection for transformation events. The new construct was compared in multiple plant species to the standard spectinomycin selectable marker gene currently used for soybean transformation and to the neomycin phosphotransferase (nptII) gene that confers kanamycin resistance. In Arabidopsis thaliana, potato, and citrus, the transformation efficiency provided by the enhanced spectinomycin marker was approximately equal to that of kanamycin and considerably better than that observed for the standard spectinomycin marker. In soybean, the enhanced spectinomycin resistance marker preformed as well as the standard spectinomycin gene. These results demonstrate that the enhanced spectinomycin construct provides strong resistance, comparable to kanamycin in multiple species, offering an alternative tool for transgenic plant selection.

Indexed as

Plants, Genetically ModifiedSpectinomycinTransformation, GeneticArabidopsisGlycine maxKanamycinKanamycin KinaseSolanum tuberosumKanamycinKanamycin KinaseSpectinomycinGene stackingKanamycin resistanceSelectable markerSpectinomycin resistanceTransgenic plants

Identifiers

PMID40121489

DOI10.1186/s13104-025-07178-3

PMCPMC11929352

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.