ArticleScientific reports2025
Quantitative detection of β-glucans in Cordyceps species using a validated Congo red assay.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Electron Beam-Degraded β-Glucans fromPolymers · 2026Article
- Research progress of polysaccharide detection methods in traditional Chinese medicine.Chinese herbal medicines · 2026Review
- Process Analysis of Millet Bran Fermentation byFoods (Basel, Switzerland) · 2026Article
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Authors and funding
8 authors.
Funding
Abstract
β-Glucan is extensively utilized in the food industry for its functional benefits, including blood glucose and lipid regulation, enhanced food texture, and a prolonged shelf life. In the pharmaceutical field, it serves as a potent immune modulator, anti-tumor agent, and vaccine adjuvant, highlighting its significant biological potential. Despite its wide-ranging applications, standardized methods for detecting β-glucan in Cordyceps species have been lacking. To address this deficiency, the current study developed a Congo Red Ultraviolet Spectrophotometry assay for β-glucan quantification. The optimal conditions for extraction were identified as dimethyl sulfoxide (DMSO) being the solvent, a pH of 7.0, a temperature of 65 °C, and a reaction time of 60 min. Validation tests confirmed the method's precision, reproducibility, and stability, demonstrating its reliability for β-glucan analysis. The assay was applied to three Cordyceps species, i.e., Cordyceps militaris, C. cicadae, and C. fumosorosea. Results revealed a marginally higher β-glucan content in C. fumosorosea compared to the other species. This efficient and cost-effective method offers a valuable tool for β-glucan analysis, with potential applications in quality control and product development for medicinal and edible fungi. Future research should extend to additional Cordyceps species and compare results with alternative analytical techniques to further enhance standardization and broaden the applicability of this method.
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