Evidence map›Paper›PMID 40120579›Full record

ArticleCell reports methods2025

An improved FLARE system for recording and manipulating neuronal activity.

Guanwei Zhou, Ruonan Li, Ola Bartolik, Yuqian Ma, Wei Wei Wan, Jennifer Meng, Yujia Hu, Bing Ye, Wenjing Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Guanwei ZhouLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Program in Chemical Biology, University of Michigan, Ann Arbor, MI, USA.
Ruonan LiLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Ola BartolikLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA.
Yuqian MaLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Wei Wei WanLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Department of Chemistry, University of Michigan, Ann Arbor, MI, USA.
Jennifer MengLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Yujia HuLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Bing YeLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA. Electronic address: bingye@umich.edu.
Wenjing WangLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Program in Chemical Biology, University of Michigan, Ann Arbor, MI, USA; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA; Department of Chemistry, University of Michigan, Ann Arbor, MI, USA. Electronic address: wenjwang@umich.edu.

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
Design of genetically encoded sensors for detecting endogenous opioid peptidesR01DA053200 · NIDA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Wenjing Wang · 2022 to 2026
$1.7M
The assembly of population coding networksR01NS128500 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TZUMIN LEE · 2023 to 2026
$1.5M
NIDA NIH HHS R01 DA053200NIH HHS P40 OD018537NINDS NIH HHS R01 NS128500
6 · The paper itself

Abstract

To address the need for methods for tagging and manipulating neuronal ensembles underlying specific behaviors, we present an improved version of FLARE, termed cytoFLARE (cytosol-expressed FLARE). cytoFLARE incorporates cytosolic tethering of a transcription factor and expression of a more sensitive pair of calcium-sensing domains. We show that cytoFLARE captures more calcium- and light-dependent signals in HEK293T cells and higher signal-to-background ratios in neuronal cultures. We further establish cytoFLARE transgenic Drosophila models and apply cytoFLARE to label activated neurons upon sensory or optogenetic stimulation within a defined time window. Notably, through the cytoFLARE-driven expression of optogenetic actuators, we successfully reactivated and inhibited neurons involved in the larval nociceptive system. Our findings demonstrate the characterization and application of time-gated calcium integrators for both recording and manipulating neuronal activity in Drosophila larvae.

Indexed as

NeuronsOptogeneticsAnimalsAnimals, Genetically ModifiedCalciumDrosophilaDrosophila melanogasterHEK293 CellsHumansLarvaCalciumbehaviorCP: NeuroscienceDrosophilaintegrator sensorneuronal activityoptogeneticstranscriptional reporter

Identifiers

PMID40120579
PMCPMC12256943

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.