Evidence map›Paper›PMID 40119535›Full record

ArticleBiotechnology and bioengineering2025

Engineering an Overflow-Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production.

Jianli Zhang, Jian Wang, Tian Jiang, Xinyu Gong, Qi Gan, Yuxi Teng, Yusong Zou, Ainoor Anwar Dawadi, Yajun Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jianli ZhangSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Jian WangSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Tian JiangSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Xinyu GongSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Qi GanSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Yuxi TengSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Yusong ZouSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Ainoor Anwar DawadiSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.
Yajun YanSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia, USA.ORCID 0000-0002-9993-3016

Funding

Engineering Dynamic Control of Natural Product Biosynthesis in BacteriaR35GM128620 · NIGMS · UNIVERSITY OF GEORGIA · PI Yajun Yan · 2018 to 2026
$3.0M
NIGMS NIH HHS R35 GM128620This study was supported by National Institute of General Medical Sciences (Grant R35GM128620).
6 · The paper itself

Abstract

Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose as carbon source. This phenomenon, known as overflow metabolism, has negative impacts on cell growth and protein expression, also causes carbon loss during biosynthesis in most microbial production scenarios. In this study, we regarded the "waste" metabolite as a useful metabolism indicator, constructed an overflow biosensor to monitor the change of acetate concentration and converted the signal into various regulation outputs. Phloroglucinol is a phenolic compound with several derivatives that exhibit various pharmacological activities. By applying the bifunctional dynamic regulation system on the phloroglucinol production, we released the cellular redox pressure in real-time and reduced the waste of carbon flux on overflow metabolism. Finally, carbon flux was redirected more favorably towards the desired product, resulting in a boosted phloroglucinol titer of 1.30 g/L, increased by 2.04-fold. Overall, this study explored the use of a central byproduct-responsive biosensor system on improving cellular metabolic status, providing a general approach for enhancing bioproduction.

Indexed as

Escherichia coliMetabolic EngineeringPhloroglucinolAcetatesBiosensing TechniquesGlucoseOxidation-ReductionAcetatesGlucosePhloroglucinolacetatebiosensorbiosynthesisdynamic regulationoverflow metabolismredox ratio

Identifiers

PMID40119535
PMCPMC12067040

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.