Evidence map›Paper›PMID 40119264›Full record

ArticleBMC genomics2025

Whole-genome bisulfite sequencing of X and Y sperm in Holstein bulls reveals differences in autosomal methylation status.

Aishao Shangguan, Fengling Ding, Rui Ding, Wei Sun, Xihe Li, Xiangnan Bao, Tiezhu Zhang, Huihui Chi, Qi Xiong, Mingxin Chen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Aishao Shangguan *Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Science and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430070, China.
Fengling Ding *Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Rui DingNational Center of Technology Innovation for Dairy, Hohhot, 010020, China.
Wei SunNational Center of Technology Innovation for Dairy, Hohhot, 010020, China.
Xihe LiNational Center of Technology Innovation for Dairy, Hohhot, 010020, China.
Xiangnan BaoNational Center of Technology Innovation for Dairy, Hohhot, 010020, China.
Tiezhu ZhangInner Mongolia SaiKeXing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, 010020, China.
Huihui ChiInner Mongolia SaiKeXing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, 010020, China.
Qi XiongHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Science and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430070, China.
Mingxin ChenHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Science and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430070, China.
Yang ZhouFrontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China. yangzhou@mail.hzau.edu.cn.
Shujun ZhangFrontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China. sjxiaozhang@mail.hzau.edu.cn.

Funding

Financial assistance from the Fundamental Research Funds for the Central Universities 2662023DKPY001Inter-Governmental International Science and Technology Cooperation Project of the State Key Research and Development Program 2021YFE0115500National Center of Technology Innovation for Dairy 2022-KYGG-3Wuhan Municipal Knowledge Innovation Special Project 2023020201010140
6 · The paper itself

Abstract

A comprehensive understanding of the molecular differences between X and Y sperm in Holstein bull semen is crucial for advancing sex control technologies. While previous studies have primarily focused on proteomic and transcriptomic differences, the genome-wide DNA methylation differences between these sperm types remains largely unexplored. In this study, we employed whole-genome bisulfite sequencing to systematically compare the autosomal methylation profiles of X and Y sperm. Although global methylation patterns showed remarkable consistency between the two sperm types, our localized comparative analysis revealed 12,175 differentially methylated regions mapping to 2,041 genes (differentially methylated genes, DMGs). Functional enrichment analysis of these DMGs revealed their involvement in essential biological processes, particularly in energy metabolism and membrane voltage regulation. Notably, SPA17 and CHCHD3, identified as hypermethylated genes in X sperm in this study, have also been reported to show lower protein expression levels in X sperm compared to Y sperm. Furthermore, we identified 28 DMGs functionally associated with spermatogenesis and 5 DMGs related to fertilization. Our findings lay the foundation for thorough understanding of molecular differences between X and Y sperm in bull, providing essential insights for the development of more advanced sex control technologies in the future.

Indexed as

DNA MethylationSpermatozoaWhole Genome SequencingX ChromosomeY ChromosomeAnimalsCattleMaleSulfiteshydrogen sulfiteSulfitesDNA methylationEpigeneticsHolstein bullWhole-genome bisulfite sequencingX sperm and Y sperm

Identifiers

PMID40119264
PMCPMC11927118

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.