Evidence map›Paper›PMID 40117313›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Decoding in-cell respiratory enzyme dynamics by label-free in situ electrochemistry.

Yoshihide Tokunou, Tomohiko Yamazaki, Takashi Fujikawa, Akihiro Okamoto

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yoshihide TokunouDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.ORCID 0000-0002-0983-6176
Tomohiko YamazakiResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan.ORCID 0000-0003-2136-8042
Takashi FujikawaResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan.ORCID 0000-0001-7734-546X
Akihiro OkamotoDepartment of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.ORCID 0000-0002-8102-4316

Funding

MEXT ARIM JPMXP1223NM5212MEXT | Japan Science and Technology Agency (JST) JPMJGX23B2MEXT | Japan Society for the Promotion of Science (JSPS) 20K15428MEXT | Japan Society for the Promotion of Science (JSPS) 22KK0242MEXT | Japan Society for the Promotion of Science (JSPS) 23K23532MEXT | JST | ACT-X JPMJAX211CMEXT | JST | Fusion Oriented REsearch for disruptive Science and Technology (FOREST) JST FORESTUniversity of Tsukuba (ç'波大å¦) None
6 · The paper itself

Abstract

Deciphering metabolic enzyme catalysis in living cells remains a formidable challenge due to the limitations of in vivo assays, which focus on enzymes isolated from respiration. This study introduces an innovative whole-cell electrochemical assay to reveal the Michaelis-Menten landscape of respiratory enzymes amid complex molecular interactions. We controlled the microbial current generation's rate-limiting step, extracting in vivo kinetic parameters (

Indexed as

Electrochemical TechniquesEscherichia coliEscherichia coli ProteinsKineticsEscherichia coli Proteinsenzymatic activityinterprotein interactionMichaelis–Menten equation

Identifiers

PMID40117313
PMCPMC11962448

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.