Evidence map›Paper›PMID 40117061›Full record

ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2025

TIMP3 deficiency accelerates UVB-induced HaCaT cell senescence by regulating ferroptosis.

Yan Teng, Junjia He, Yeyu Shen, Jie Chen, Ye Qian, Youming Huang, Xiaohua Tao, Danfeng Xu, Yibin Fan

Abstract read
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In one paragraph

Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yan TengCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China.
Junjia HeCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China.
Yeyu ShenCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China.
Jie ChenZhuji Sixth People's Hospital, Zhuji, Zhejiang, People's Republic of China.
Ye QianDepartment of Gastroenterology, Chun'an County First People's Hospital (Zhejiang Provincial People's Hospital, Chun'an Branch), Hangzhou, Zhejiang, People's Republic of China.
Youming HuangCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China.
Xiaohua TaoCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China.
Danfeng XuCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China. 3100102508@zju.edu.cn.
Yibin FanCenter for Plastic and Reconstructive Surgery, Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, 310014, Zhejiang, People's Republic of China. fanyibin@hmc.edu.cn.

Funding

National Natural Science Foundation of China 82073453Zhejiang Medicine and Health Research Fund Project 2022RC099Zhejiang Medicine and Health Research Fund Project 2024KY650Zhejiang Medicine and Health Research Fund Project 2024KY750
6 · The paper itself

Abstract

Prolonged exposure to ultraviolet B (UVB) light leads to the accumulation of reactive oxygen species (ROS), a key contributor to skin aging. Previous studies have demonstrated that UVB exposure results in a deficiency in the expression of TIMP3 in keratinocytes. The objective of this study was to investigate the specific role of TIMP3 in keratinocytes. UVB-treated HaCaT cells were utilized to establish a cellular photoaging model. We found that UVB significantly increased levels of ROS, promoted senescence and ferroptosis, and inhibited the expression of TIMP3 in HaCaT. This inhibition was notably alleviated by Fer-1, a ferroptosis inhibitor. In addition, the knockdown of TIMP3 in HaCaT enhanced senescence by inducing the ferroptosis. Mechanistically, UVB exposure also led to a decrease in the expression of KLF4, a transcription factor that regulated TIMP3 expression. Futhermore, UVB-induced reduced expression of KLF4 and TIMP3 in vivo. Our results suggest that deletion of the KLF4/TIMP3 axis promotes HaCaT cell senescence by facilitating the progression of ferroptosis. TIMP3 may serve as an effective therapeutic target for preventing skin photoaging.

Indexed as

Cellular SenescenceFerroptosisTissue Inhibitor of Metalloproteinase-3Ultraviolet RaysAnimalsHaCaT CellsHumansKeratinocytesKruppel-Like Factor 4Kruppel-Like Transcription FactorsReactive Oxygen SpeciesKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription FactorsReactive Oxygen SpeciesTIMP3 protein, humanTissue Inhibitor of Metalloproteinase-3FerroptosisKLF4ROSSkin senescenceTIMP3

Identifiers

PMID40117061

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.