ArticleAnalytical and bioanalytical chemistry2025
Digital PCR assays for quantifying trichothecene-producing Fusarium species, including Fusarium langsethiae, F. poae, and F. sporotrichioides, in oats.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Revisiting the PCR-Based Molecular Approaches for Mycotoxigenic Fusarium Species Detection and Quantification in Wheat.Comprehensive reviews in food science and food safety · 2026Review
- From Morphology to Multi-Omics: A New Age of Fusarium Research.Pathogens (Basel, Switzerland) · 2025Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Fusarium fungi cause Fusarium head blight (FHB) in oats, reducing yield and contaminating grains with harmful trichothecene mycotoxins. FHB symptoms in oats are often not visually distinct, necessitating alternative detection methods. We developed digital PCR (dPCR) assays as the most accurate DNA-based method to detect trichothecene-producing Fusarium species commonly found in oats. Building on existing quantitative PCR (qPCR) assays, we developed dPCR assays targeting all trichothecene producers (the Tri5 gene), or specific to F. langsethiae (Fl), F. poae (Fp), and F. sporotrichioides (Fs). All targeted single copy genes, except F. poae which targeted rDNA which is a variable and multi-copy target (and hence not as reliable as the other assays for quantification). Optimized dPCR assays showed excellent linearity (R
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