ArticleCurrent protocols2025
Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples.Current protocols · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
High concentrations of aerosols can be generated within the sort collection area of cell sorters during instrument failures that cause the stream to deviate, such as a partial nozzle obstruction. Complete containment of these aerosol particles becomes essential for operator safety when working with potentially infectious or hazardous samples. Currently, aerosol containment is accomplished through the generation of continuous negative airflow within the sort collection area using an aerosol evacuation system, which can be enhanced by using primary containment devices such as biosafety cabinets. Unlike biosafety cabinets, many aerosol evacuation systems are not certified or tested on a regular basis after installation. Therefore, proper function of the system must be verified by the user prior to running hazardous samples to ensure that it is operational and provides sufficient protection for the operator. This protocol describes an updated procedure for verifying the containment and evacuation of aerosols generated when the stream is disrupted during an instrument failure. In this procedure, aerosols are generated to simulate a partial nozzle obstruction while running 1-µm fluorescent beads. Air samples are collected just outside the sort collection area using a disposable impactor-style aerosol sampler cassette and are examined for the presence of beads in an effort to detect aerosols. If no beads are present, aerosols were adequately contained and evacuated by the aerosol evacuation system. The presence of beads, however, indicates a potential failure of the aerosol evacuation system and/or other engineering controls that could result in the exposure of laboratory workers to any infectious or hazardous samples that are run through the instrument. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. Basic Protocol: Validation of the aerosol evacuation system using 1-µm fluorescent beads and disposable aerosol sampler cassettes Support Protocol: Preparation of 1-µm fluorescent bead reference slide.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.