ArticleCureus2025
Mapping Viral Landscapes: Genomic Surveillance of Aedes Mosquitoes From Central India.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundMosquito-borne viral diseases pose a significant impact on human health, with the potential to cause widespread outbreaks of diseases. Monitoring viral genomes in mosquito populations can lead to informed risk assessment and promote early diagnosis. However, a standardized methodology is lacking to decipher circulating viral sequences in mosquito populations collected from human habitats. Our study aims to establish and evaluate a system of viral metagenomic analysis in the
methodsMosquitoes were collected using CDC-approved BG-Sentinel version 2 traps (Biogents AG, Regensburg, Germany) and battery-operated vacuum aspirators from different locations in the Bhopal region, India. They were sorted based on genus, gender, location, and collection date. The RNA was extracted from the homogenized mosquito pools and reverse transcribed. Complementary DNA (cDNA) was amplified using sequence-independent, single-prime amplification (SISPA). Further, polymerase chain reaction (PCR) products were sequenced using the Illumina NovaSeq 6000 platform (Illumina, Inc., San Diego, CA). Bioinformatic analysis of the reads was performed using Trimmomatic (Bolger AM, Lohse M, Usadel B (2014). Trimmomatic: A flexible trimmer for Illumina Sequence Data (Bioinformatics, btu170) for trimming low-quality raw reads. Later, Kraken2 and Bracken (Johns Hopkins University, Baltimore, MD) were used for the identification of viral sequences.
resultsThe study examined virus diversity and seasonal distribution in mosquito populations collected from 13 sites in Bhopal, India, over 15 months. A total of 31 mosquito pools of female
conclusionsOur findings demonstrate the potential of combining entomological and genomic surveillance for monitoring virus circulation in mosquito populations, which may be implemented as a routine surveillance tool for the timely detection of spikes in viruses with human pathogenic potential and thus inform targeted vector control measures to avert potential outbreaks in the future.
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