Evidence map›Paper›PMID 40114516›Full record

ArticleACS synthetic biology2025

Thylakoid Targeting Improves Stability of a Cytochrome P450 in the Cyanobacterium

Sayali S Hanamghar, Silas Busck Mellor, Lisbeth Mikkelsen, Christoph Crocoll, Mohammed Saddik Motawie, David A Russo, Poul Erik Jensen, Julie A Z Zedler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Optimising COMicrobial cell factories · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sayali S HanamgharSynthetic Biology of Photosynthetic Organisms, Matthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, 07743 Jena, Germany.
Silas Busck MellorDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.
Lisbeth MikkelsenDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.
Christoph CrocollDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.
Mohammed Saddik MotawieDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark.ORCID 0000-0001-5582-9463
David A RussoBioorganic Analytics, Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany.ORCID 0000-0002-4729-1701
Poul Erik JensenDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg, Denmark.
Julie A Z ZedlerSynthetic Biology of Photosynthetic Organisms, Matthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, 07743 Jena, Germany.ORCID 0000-0002-0462-8810

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plants produce a large array of natural products of biotechnological interest. In many cases, these compounds are naturally produced at low titers and involve complex biosynthetic pathways, which often include cytochrome P450 enzymes. P450s are known to be difficult to express in traditional heterotrophic chassis. However, cyanobacteria have shown promise as a sustainable alternative for the heterologous expression of P450s and light-driven product biosynthesis. In this study, we explore strategies for improving plant P450 stability and membrane insertion in cyanobacteria. The widely used model cyanobacterium

Indexed as

Cytochrome P-450 Enzyme SystemSynechocystisThylakoidsBacterial ProteinsNitrilesOximesSorghumBacterial ProteinsCytochrome P-450 Enzyme SystemdhurrinNitrilesOximescyanobacteriaCYP79A1cytochrome P450light-driven catalysisPetC1p-hydroxyphenylacetaldoximeRieske proteinSynechocystis sp. PCC 6803

Identifiers

PMID40114516
PMCPMC11934225

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.