Evidence map›Paper›PMID 40114082›Full record

ArticleBMC genomics2025

Abnormal DNA methylation of EBF1 regulates adipogenesis in chicken.

Xiaohuan Chao, Lijin Guo, Meiling Hu, Mao Ye, Zhexia Fan, Kang Luan, Jiahao Chen, Chunlei Zhang, Manqing Liu, Bo Zhou and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xiaohuan ChaoState Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, China.
Lijin GuoCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Meiling HuGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China.
Mao YeCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Zhexia FanCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Kang LuanCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Jiahao ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Chunlei ZhangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Manqing LiuCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Bo ZhouCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Xiquan ZhangCollege of Animal Science, South China Agricultural University, Guangzhou, China.
Zhenhui LiCollege of Animal Science, South China Agricultural University, Guangzhou, China. lizhenhui@scau.edu.cn.
Qingbin LuoCollege of Animal Science, South China Agricultural University, Guangzhou, China. qbluo@scau.edu.cn.

Funding

Science and Technology Program of Chaozhou City No.202101ZD07Science and Technology Program of Guangzhou City No. 2024B03J1353The Key-Area Research and Development Program of Guangdong Province No.2022B0202100002
6 · The paper itself

Abstract

backgroundDNA methylation influences gene expression and is involved in numerous biological processes, including fat production. It is involved in lipid generation in numerous animal species, including poultry. However, the effect of DNA methylation on adipogenesis in chickens remains unclear.

resultsA total of 12 100-day-old chickens were divided into high and low-fat groups based on their abdominal fat ratios. Subsequently, genome-wide bisulfite sequencing (WGBS) was performed on their abdominal fat, and 1877 differentially methylated region (DMR) genes were identified, among which SLC45A3, EBF1, PLA2G15, and ACAD9 were associated with lipid metabolism. Interestingly, EBF1 showed a lower level of DNA methylation and higher mRNA expression in the low-fat group, as determined by comprehensive RNA-seq analysis. Cellular verification showed that EBF1 expression was upregulated by 5-azacytidine (5-Aza) and downregulated by betaine. EBF1 facilitated the differentiation of immortalized chicken preadipocyte 1 (ICP-1) through the PPAR-γ pathway, thereby affecting chicken adipogenesis.

conclusionA combination of WGBS and RNA-seq analyses revealed 48 DMGs in the abdominal fat tissue of chickens. Notably, the DNA methylation status of EBF1 was inversely related to its mRNA expression. Mechanistically, DNA methylation regulates EBF1 expression, which in turn mediates the differentiation of ICP-1 through the PPARγ pathway. This study provides a theoretical framework for investigating the effects of DNA methylation on adipogenesis in chickens.

Indexed as

AdipogenesisChickensDNA MethylationTrans-ActivatorsAbdominal FatAnimalsCell DifferentiationPPAR gammaPPAR gammaTrans-ActivatorsAdipogenesisDMGsDNA methylationEBF1WGBS

Identifiers

PMID40114082
PMCPMC11927125

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.