Evidence map›Paper›PMID 40113733›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2025

Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging.

Yusuke Murakami, Mia Obuchi, Hiroshi Kamizawa, Shinichi Miyazaki, Akihiro Kishimura, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Yu Hayashi, Kentaro Shiraki and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yusuke MurakamiPh. D. Program in Humanics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Mia ObuchiDepartment of Chemistry, Faculty of Science, Kyushu University, 744 Moto-Oka, Nishi-Ku, Fukuoka, 819-0395, Japan.
Hiroshi KamizawaGraduate School of System Life Sciences, Kyushu University, 744 Moto-Oka, Nishi-Ku, Fukuoka, 819-0395, Japan.
Shinichi MiyazakiInternational Institute for Integrative Sleep Medicine (WPI-IIIS), 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Akihiro KishimuraDepartment of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Moto-Oka, Nishi-Ku, Fukuoka, 819-0395, Japan.
Ryosuke OketaniDepartment of Chemistry, Faculty of Science, Kyushu University, 744 Moto-Oka, Nishi-Ku, Fukuoka, 819-0395, Japan.
Kotaro HiramatsuDepartment of Chemistry, Faculty of Science, Kyushu University, 744 Moto-Oka, Nishi-Ku, Fukuoka, 819-0395, Japan.
Philippe LeprouxInstitut de Recherche XLIM, UMR CNRS No. 7252, 123 Avenue Albert Thomas, 87060, Limoges CEDEX, France.
Yu HayashiInternational Institute for Integrative Sleep Medicine (WPI-IIIS), 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Kentaro ShirakiFaculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan.
Hideaki KanoFaculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-Ku, Yokohama, Kanagawa, 223-8522, Japan. hkano@bio.keio.ac.jp.ORCID http://orcid.org/0000-0003-3682-7627

Funding

Agence Nationale de la Recherche ANR-10-LABX-0074 Sigma-LIMJapan Society for the Promotion of Science London 18H02383Japan Society for the Promotion of Science London 21H04961Japan Society for the Promotion of Science London 23K17933Japan Society for the Promotion of Science London 23KJ1721Japan Society for the Promotion of Science London 24H01151JST-Mirai Program JPMJMI22G5
6 · The paper itself

Abstract

Liquid-liquid phase separation leads to the formation of liquid droplets (LqDs) such as P granules in Caenorhabditis elegans (C. elegans). In this study, we demonstrate the label-free visualization of LqDs using multimodal nonlinear optical imaging both in vitro and in vivo. In vitro measurements with polymerized adenine [poly(A)], we found significantly higher poly(A) concentrations in LqDs compared to surrounding solutions, with the limit of detection (LoD) of 32 mg/mL. In vivo measurements, we performed label-free imaging of C. elegans. Despite efforts to detect P granules within P lineage cells in both wild-type C. elegans and green fluorescent protein (GFP)-tagged strains, no clear RNA-specific signals were observed. This indicates that the RNA concentration in P granules is lower than anticipated and falls below our in vitro LoD. These results underscore the challenges of label-free RNA detection in P granules.

Indexed as

Caenorhabditis elegansNonlinear DynamicsOptical ImagingAnimalsPhase SeparationPoly APoly ACaenorhabditis elegansCoherent anti-Stokes Raman scattering (CARS)Liquid–liquid phase separationLLPSMicroscopyP granulesSpectroscopic imaging

Identifiers

PMID40113733

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.