ArticleMicrobial biotechnology2025
Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.
Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Biomanufacturing and Scale-Up: Pathways to Biochemicals, Biofuels, and Biomaterials.Microbial biotechnology · 2026Article
- Defined YNB-free mineral medium improves reproducibility and enables high-titer production in Yarrowia lipolytica.Microbial cell factories · 2026Article
- Fatty acid synthesis: A critical factor determining mycelial growth rate inFood chemistry. Molecular sciences · 2025Article
- Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools.Microbial biotechnology · 2025Review
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation and loss of biodiversity, thus sustainable alternatives are required. Microbial oils, especially from Yarrowia lipolytica, offer a promising solution because of their similar composition to palm oil, low carbon footprint and ability to utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach to enhance lipid production in Y. lipolytica. We systematically evaluated predictions from the genome-scale metabolic model to identify and overcome bottlenecks in lipid biosynthesis. We tested the effect of predicted medium supplements (glutamate, leucine, methionine and threonine) and genetic intervention targets, including the overexpression of ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC), threonine synthase (TS), diacylglycerol acyltransferase(DGA1), the deletion of citrate exporter gene (CEX1) and disruption of β-oxidation pathway (MFE1). This work revealed the critical roles of ACC, ACL, TS and DGA1 and the interaction of these genes with elevated intracellular citrate availability in lipid biosynthesis. Combining TS and DGA1 overexpression in the Δmfe_Δcex background achieved a remarkable 200% increase in lipid content (56% w/w) and a 230% increase in lipid yield on glycerol. These findings underscore the potential of Y. lipolytica as an efficient microbial cell factory for fatty acid production. Our study advances the understanding of lipid metabolism in Y. lipolytica and demonstrates a viable approach for developing sustainable and economically feasible alternatives to palm oil.
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Registered trials
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