Evidence map›Paper›PMID 40113630›Full record

ArticleArchives of microbiology2025

The importance role of central proline to the antimicrobial potency and selectivity of indolicidin.

Hang Ngo Thu, Hoa Ngo Van, Hai Bui Thi Phuong, Thinh Pham Duc, Khanh Hoang Van, Khoa Nguyen Vu, Tan Nguyen Van, Linh Dang Thuy, Le Minh Bui, Tung Truong Thanh and 2 more

Abstract read
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Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Nanosystems for delivery of indolicidin peptide.Frontiers in medical technology · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hang Ngo ThuDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Hoa Ngo VanMilitary Hospital 103, Vietnam Military Medical University, 12108, Hanoi, Vietnam.
Hai Bui Thi PhuongFaculty of Pharmacy, PHENIKAA University, Hanoi, 12116, Vietnam.
Thinh Pham DucPharmaceutical Training Institute, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Khanh Hoang VanDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Khoa Nguyen VuDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Tan Nguyen VanDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Linh Dang ThuyDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam.
Le Minh BuiDepartment of Biotechnology, NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh, 700000, Vietnam.
Tung Truong ThanhFaculty of Pharmacy, PHENIKAA University, Hanoi, 12116, Vietnam.
Huy Luong XuanFaculty of Pharmacy, PHENIKAA University, Hanoi, 12116, Vietnam.
Mao Can VanDepartment of Pathophysiology, Vietnam Military Medical University, Hanoi, 12108, Vietnam. canvanmao@vmmu.edu.vn.

Funding

Ministry of National Defence (Vietnam) 2022.75.50
6 · The paper itself

Abstract

The increasing prevalence of multidrug-resistant pathogens urged the development of new therapeutic strategies, and antimicrobial peptides (AMPs) have emerged as promising candidates. Indolicidin, a proline-rich AMP, is effective against a wide range of pathogens by penetrating the membrane to disrupt the cytoplasmic membrane or inhibit DNA synthesis. This study investigates the impact of replacing the central proline residue in Indolicidin with glycine (IND-7G), D-proline (IND-7DP), or lysine (IND-7K). Results show that both glycine and D-proline substitutions significantly reduced antimicrobial activity with lower hemolysis than the parent peptide. Besides, the analog having lysine substitution (IND-7K) slightly increased activity against E. coli and C. albicans but reduced potency against S. aureus, E. faecalis, and K. pneumoniae. The hemolytic activity of IND-7K remained comparable to that of Indolicidin. These findings demonstrated the essential role of proline in maintaining the antimicrobial efficacy of Indolicidin.

Indexed as

Anti-Bacterial AgentsAnti-Infective AgentsAntimicrobial Cationic PeptidesProlineCandida albicansEscherichia coliHemolysisHumansKlebsiella pneumoniaeMicrobial Sensitivity TestsStaphylococcus aureusAnti-Bacterial AgentsAnti-Infective AgentsAntimicrobial Cationic PeptidesindolicidinProlineAntimicrobial peptidesIndolicidinProline-rich peptidesProline substitutionStructure–activity relationships

Identifiers

PMID40113630

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.