Evidence map›Paper›PMID 40113441›Full record

ArticlePhysiologia plantarum

A suitable strategy to find IAA metabolism mutants.

Rubén Casanova-Sáez, Aleš Pěnčík, Rafael Muñoz-Viana, Federica Brunoni, Rui Pinto, Ondřej Novák, Karin Ljung, Eduardo Mateo-Bonmatí

Abstract read
In one paragraph

Article in Physiologia plantarum. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rubén Casanova-SáezUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0001-5683-7051
Aleš PěnčíkUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0002-1314-2249
Rafael Muñoz-VianaUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0002-1363-6978
Federica BrunoniUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0003-1497-9419
Rui PintoComputational Life Science Cluster (CLiC), Chemistry department (KBC), Umeå University, Umeå, Sweden.ORCID https://orcid.org/0000-0002-8527-4873
Ondřej NovákLaboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of Sciences & Faculty of Science, Palacký University, Olomouc, Czech Republic.ORCID https://orcid.org/0000-0003-3452-0154
Karin LjungUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0003-2901-189X
Eduardo Mateo-BonmatíUmeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.ORCID https://orcid.org/0000-0002-2364-5173

Funding

Agencia Estatal de Investigación PID2023-147737NA-I00Agencia Estatal de Investigación RYC2021-030895-ICarl Tryggers foundation CTS 12:289Carl Tryggers foundation CTS 13:275Kempestiftelserna JCK-1111Kempestiftelserna JCK-1811Palacký University Olomouc IGA_PrF_2024_013The Knut and Alice Wallenberg Foundation KAW 2016.0352Vetenskapsrådet 2014-04514
6 · The paper itself

Abstract

Indole-3-acetic acid (IAA), the most common form of auxin, is involved in a great range of plant physiological processes. IAA is synthesized from the amino acid tryptophan and can be transported and inactivated in a myriad of ways. Despite intense research efforts, there are still dark corners in our comprehension of IAA metabolism and its interplays with other pathways. Genetic screens are a powerful tool for unbiasedly looking for new players in a given biological process. However, pleiotropism of auxin-related phenotypes and indirect effects make it necessary to incorporate additional screening steps to specifically find mutants affected in IAA homeostasis. We previously developed and validated a high-throughput methodology to simultaneously quantify IAA, key precursors, and inactive forms from as little as 10 mg of fresh tissue. We have carried out a genetic screening to identify mutants involved in IAA metabolism. Auxin reporters DR5

Indexed as

ArabidopsisIndoleacetic AcidsMutationArabidopsis ProteinsGene Expression Regulation, PlantPhenotypePlant Growth RegulatorsArabidopsis Proteinsindoleacetic acidIndoleacetic AcidsPlant Growth Regulators

Identifiers

PMID40113441
PMCPMC11925725

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.