Evidence map›Paper›PMID 40111611›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Functional Evaluation of Astrocytic Responses to Stress.

Lewis Depaauw-Holt, Sarah Peyrard, Anthony Bosson, Ciaran Murphy-Royal

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lewis Depaauw-HoltDépartement de Neurosciences, Université de Montréal, Montréal, QC, Canada.
Sarah PeyrardCentre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada.
Anthony BossonCentre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada.
Ciaran Murphy-RoyalDépartement de Neurosciences, Université de Montréal, Montréal, QC, Canada. ciaran.murphy-royal@umontreal.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In order to fully understand the contribution of astrocytes to stress-related psychiatric disorders, functional assays are required. There are numerous approaches that can be used to study astrocyte function including live-cell imaging of surface protein trafficking, patch-clamp recording of glutamate transporter currents, or calcium imaging using inorganic dyes and genetically encoded calcium indicators. Here, we will outline a method to record and analyze calcium activity in astrocytes using virus-mediated expression of the genetically encoded calcium indicator GCaMP. This approach gives insight into specific astrocyte changes in response to stress which could include hyper- or hypo-activity in these cells.

Indexed as

AstrocytesStress, PhysiologicalAnimalsCalciumCalcium SignalingCells, CulturedHumansPatch-Clamp TechniquesCalciumAstrocytesCalcium imagingEarly-life stressLive-cell imagingStress

Identifiers

PMID40111611

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.