Evidence map›Paper›PMID 40111610›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Morphological Investigation of Astrocytic Responses to Stress.

Lewis Depaauw-Holt, Sarah Peyrard, Anthony Bosson, Ciaran Murphy-Royal

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lewis Depaauw-HoltDépartement de Neurosciences, Université de Montréal, Montréal, QC, Canada.
Sarah PeyrardCentre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada.
Anthony BossonCentre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, QC, Canada.
Ciaran Murphy-RoyalDépartement de Neurosciences, Université de Montréal, Montréal, QC, Canada. ciaran.murphy-royal@umontreal.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There are many ways in which astrocytes are likely to respond to stress, but one of the most reliable phenotypes has been glial fibrillary acidic protein (GFAP) and morphological changes. GFAP is usually a reliable indicator for morphological reorganization but cannot be used alone for detailed morphological reconstruction and analysis. Sparse labeling of astrocytes with a fluorescent indicator, e.g., eGFP, is a robust way to determine discrete morphological changes in these cells. Here, we outline both methods to study stress-induced changes in astrocyte morphology.

Indexed as

AstrocytesStress, PhysiologicalAnimalsCells, CulturedGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsMiceenhanced green fluorescent proteinGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsAstrocytesCellular imagingEarly-life stressGFAPImmunohistochemistryMorphologySingle-cell morphologyStress

Identifiers

PMID40111610

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.