Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers.

Carmen Muñoz-Ballester, Samantha Golf, Benjamin Heithoff, Kijana George, Stefanie Robel

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Carmen Muñoz-BallesterDepartment of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

Samantha GolfDepartment of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

Benjamin HeithoffFralin Biomedical Research Institute at Virginia Tech Carilion, Roanoke, VA, USA.

Kijana GeorgeFralin Biomedical Research Institute at Virginia Tech Carilion, Roanoke, VA, USA.

Stefanie RobelDepartment of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA. srobel@uab.edu.

Funding

Dynamic temporal regulation of astrocyte coupling to shape neuronal activity during acquired epilepsy developmentR01NS121145 · NINDS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Stefanie Robel · 2022 to 2026

$1.8M

NINDS NIH HHS R01 NS121145

6 · The paper itself

Abstract

Under physiological conditions, the blood-brain barrier (BBB) is required for regulating the passage of substances between the bloodstream and the brain. To date, disruption of the BBB has been associated with nearly all neurological diseases. Dye and tracer experiments have been used to assess BBB integrity for decades, yet quantitative assessment of these experiments has only recently been implemented. Further, interpretation of these results requires the consideration of tracer-specific properties. Here, we describe a protocol for evaluating BBB leakage using the small molecule cadaverine in a quantitative way. We also cover advantages/disadvantages of using some reagents and highlight limitations in data interpretations based on assays used to assess BBB permeability.

Indexed as

Blood-Brain BarrierColoring AgentsFluorescent DyesAnimalsCapillary PermeabilityHumansColoring AgentsFluorescent DyesBBBBlood–brain barrierCadaverineDyeImpairmentLeakagePermeabilityTracer

Identifiers

PMID40111602

DOI10.1101/pdb.prot087684

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.