Evidence map›Paper›PMID 40111540›Full record

ArticleJournal of gastroenterology2025

Myeloid-derived MANF ameliorates ethanol-induced liver injury by enhancing microRNA-223 expression.

Huiyuan Xie, Pingping Zhang, Shanru Yang, Jia Du, Yan Ren, Xianxian Gao, Na Li, Tao Yang, Yang Ma, Xin Hou

Abstract read
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In one paragraph

Article in Journal of gastroenterology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huiyuan Xie *Department of Laboratory Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, 315010, Zhejiang, China.
Pingping Zhang *Institute of Clinical Pharmacology, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Anhui Medical University, Hefei, 230032, Anhui, China.
Shanru YangHealth Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China.
Jia DuInstitute of Clinical Pharmacology, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Anhui Medical University, Hefei, 230032, Anhui, China.
Yan RenHealth Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China.
Xianxian GaoHealth Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China.
Na LiHealth Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China.
Tao YangDepartment of General Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Yang MaInstitute of Clinical Pharmacology, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Anhui Medical University, Hefei, 230032, Anhui, China. mayang_ahmu@126.com.
Xin HouHealth Science Center, Ningbo University, Ningbo, 315211, Zhejiang, China. houxin@nbu.edu.cn.ORCID 0000-0002-5980-5925

Funding

National Natural Science Foundation of China 82170603National Natural Science Foundation of China 82470624Natural Science Foundation of Ningbo Municipality 2024J033
6 · The paper itself

Abstract

backgroundMyeloid cells play a pivotal role in the pathogenesis of alcoholic liver disease (ALD), yet the mechanisms regulating their function and specific contributions to ALD remain inadequately understood. This study aims to investigate the role of mesencephalic astrocyte-derived neurotrophic factor (MANF) in the development of ALD.

methodsMyeloid-specific Manf knockout mice and wild-type controls were fed an ethanol-based diet for 10 days, followed by a single ethanol binge. Hepatic MANF levels, along with the correlation between MANF and inflammatory factors in patients with alcoholic hepatitis, were analyzed using the GSE28619 dataset.

resultsOur study demonstrated that myeloid MANF expression in the liver was upregulated following chronic-plus-binge ethanol exposure. Deletion of the Manf gene in myeloid cells, including neutrophils, exacerbated ethanol-induced liver injury, steatosis, neutrophil infiltration, and reactive oxygen species production. Mechanistic analysis revealed that MANF promotes neutrophil miR-223 expression, a key anti-inflammatory factor in these cells. MANF enhances miR-223 transcription by increasing the expression of the transcription factor PU.1 via p38 mitogen-activated protein kinase signaling. In addition, hepatic MANF levels were elevated in patients with alcoholic hepatitis and correlated with IL-6, IL-1β, and phagocytic oxidase (phox) p47

conclusionMyeloid-derived MANF mitigates alcohol-induced liver injury by upregulating the neutrophilic p38-PU.1-miR-223 axis.

Indexed as

Liver Diseases, AlcoholicMicroRNAsMyeloid CellsNerve Growth FactorsAnimalsDisease Models, AnimalEthanolHepatitis, AlcoholicHumansLiverMaleMiceMice, Inbred C57BLMice, KnockoutNeutrophilsReactive Oxygen SpeciesEthanolMANF protein, mouseMicroRNAsMIR223, humanMIRN223 microRNA, mouseNerve Growth FactorsReactive Oxygen SpeciesAlcohol-associated liver diseaseMANFMicroRNA-223NeutrophilsP38 MAPK

Identifiers

PMID40111540

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