Evidence map›Paper›PMID 40110881›Full record

ArticleEpilepsia2025

Toward molecular phenotyping of temporal lobe epilepsy by spatial omics.

Isabeau Vermeulen, Ronny Mohren, Micca Neusinger, Tobias A Dancker, Michiel Vandenbosch, Jan Beckervordersandforth, Benjamin Balluff, Rianna P Van der Hel, Olaf E M G Schijns, Govert Hoogland and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epilepsia, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Isabeau VermeulenMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.ORCID https://orcid.org/0000-0001-8355-9704
Ronny MohrenMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.
Micca NeusingerMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.
Tobias A DanckerMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.ORCID https://orcid.org/0009-0003-1635-361X
Michiel VandenboschMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.
Jan BeckervordersandforthGROW-School for Oncology and Reproduction, Maastricht University, Maastricht, the Netherlands.
Benjamin BalluffMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.
Rianna P Van der HelDepartment of Neurosurgery, Maastricht University Medical Center, Maastricht, the Netherlands.
Olaf E M G SchijnsDepartment of Neurosurgery, Maastricht University Medical Center, Maastricht, the Netherlands.
Govert HooglandDepartment of Neurosurgery, Maastricht University Medical Center, Maastricht, the Netherlands.
Kim RijkersDepartment of Neurosurgery, Maastricht University Medical Center, Maastricht, the Netherlands.
Berta Cillero-PastorMaastricht MultiModal Molecular Imaging Institute, Maastricht University, Maastricht, the Netherlands.ORCID https://orcid.org/0000-0002-7407-1165
ACE epilepsy surgery group

Funding

Dutch Province of Limburg under the LINK program as part of the M4i research program and by LINK 2.0, as part of the MERLN research program
6 · The paper itself

Abstract

objectiveIn temporal lobe epilepsy (TLE), detection of the epileptogenic zone predicts a good surgical outcome. When submitted to

methodsNeocortical and hippocampal biopsies of TLE patients (n = 3 PET+, n = 3 PET-) and nonepileptic postmortem controls (n = 3) were analyzed for lipid distribution using mass spectrometry imaging (MSI). Laser capture microdissection of the neocortical gray matter and hippocampal cornu ammonis and dentate gyrus was guided by MSI-derived lipid profiles and histological annotations. Dissected areas were then subjected to liquid chromatography- tandem mass spectrometry-based label-free quantitative proteomic analysis.

resultsMSI showed distinct lipid profiles, namely, phosphatidylserines were more abundant in PET+ samples in both the neocortex and hippocampus. Proteomic analysis showed significant differences between TLE and nonepileptic postmortem controls involving pathways in neuron excitability and neurotransmitter transporters, which were upregulated in TLE. Compared to PET-, all PET+ specimens displayed significantly dysregulated calcium signaling. Additionally, the neocortex of PET+ patients showed a shift from mitochondrial to cytosolic (cytoplasm of the cell) processes, whereas the hippocampus was characterized by a disruption of glycosylation and polyamine metabolism. SIGNIFICANCE: The applied spatial omics approach demonstrated localized molecular differences between metabolic subtypes of TLE patients. These findings may further specify these TLE subtypes and provide leads for targeted treatment.

Indexed as

Epilepsy, Temporal LobeHippocampusAdultFemaleFluorodeoxyglucose F18HumansMaleMiddle AgedNeocortexPhenotypePositron-Emission TomographyProteomicsYoung AdultFluorodeoxyglucose F18lipidomicsMALDI‐MSImetabolismproteomicstemporal lobe epilepsy

Identifiers

PMID40110881
PMCPMC12290997

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.