Evidence map›Paper›PMID 40109543›Full record

ArticleBiomedical optics express2025

Rapid and cost-effective screening of therapeutic targets for isoquercitrin in insulin resistance using virtual methods and fiber SPR biosensing.

Yong Wei, Yuye Xuan, Wenxiang Wang, Yonghui Zhang, Xiaoshan Li, Chunlan Liu, Chen Wang, Zhihai Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yong WeiCollege of Electronic and Information Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
Yuye XuanCollege of Electronic and Information Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
Wenxiang WangChongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Three Gorges Medical College, Chongqing 404120, China.
Yonghui ZhangChongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Three Gorges Medical College, Chongqing 404120, China.
Xiaoshan LiChongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Three Gorges Medical College, Chongqing 404120, China.
Chunlan LiuCollege of Electronic and Information Engineering, Chongqing Three Gorges University, Chongqing 404100, China.ORCID https://orcid.org/0000-0001-7281-0123
Chen WangChongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Three Gorges Medical College, Chongqing 404120, China.
Zhihai LiuKey Laboratory of In-fiber Integrated Optics, Ministry Education of China, Harbin Engineering University, Harbin 150001, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The existing screening methods for therapeutic targets of active ingredients in traditional Chinese medicine (TCM) have problems of long detection time and high instrument cost. This article proposes a new target screening method based on virtual screening and fiber surface plasmon resonance (SPR) sensing technology, which has the characteristics of flexibility, speed, and low cost. It also reveals the target mechanism of the active ingredient isoquercitrin in the treatment of insulin resistance (IR). The binding energies of isoquercitrin with target proteins PDPK1, INSR, and PTPN1 were calculated using computer virtual methods to be -8.9, -8.9, -8.8 kcal/mol, indicating strong binding activity with isoquercitrin and predicted as three key targets. Then a fiber optic SPR biosensor functionalized with isoquercitrin molecules was constructed to detect the binding affinity between isoquercitrin and the key targets. The experimental results showed that the binding affinities of isoquercitrin to the targets PDPK1, INSR, and PTPN1 were 1.45, 1.14, and 13.21, respectively, indicating that PTPN1 is the main target of isoquercitrin in the treatment of IR. The proposed sensor has a sensitivity of 0.699 nm/(μg/ml), LOD of 0.515μg/ml, and the experimental detection time of this method is as low as 45 minutes, without the need for large and expensive optical demodulation equipment, and the device volume is 5.50 dm

Identifiers

PMID40109543
PMCPMC11919358

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