ArticleScientific reports2025
Bio-nanoparticles sensor couple with smartphone digital image colorimetry and dispersive liquid-liquid microextraction for aflatoxin B1 detection.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A novel nanobiosensor-based colorimetric method was developed by integrating ZnO nanoparticles functionalized with curcumin, dispersive liquid-liquid microextraction (DLLME), and smartphone digital image colorimetry for the sensitive detection of aflatoxin B1 (AFB1) in baby food samples. The unique combination of biologically-derived ZnO nanoparticles with curcumin created a sensing platform, while DLLME provided efficient pre-concentration of the target analyte. A custom-designed portable colorimetric box enabled standardized image capture and analysis using a smartphone camera and colorimetric software. Under optimized conditions using chloroform as the extraction solvent and acetonitrile as the disperser solvent, the method achieved a remarkable limit of detection of 0.09 μg/kg within linear concentration range of 0-1 μg/L. The calibration curves demonstrated excellent linearity (R
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