Evidence map›Paper›PMID 40105452›Full record

ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2025

Localized Knockout of E-Cadherin in Subglottic Mucosa Increases Fibrosis.

Raymond J So, Samuel L Collins, Yee Chan-Li, Ioan Lina, Alexander Gelbard, Kevin M Motz, Alexander T Hillel

Abstract read
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In one paragraph

Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Raymond J SoDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0003-1004-7606
Samuel L CollinsDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Yee Chan-LiDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Ioan LinaDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Alexander GelbardDepartment of Otolaryngology-Head and Neck Surgery, Vanderbilt University, Nashville, Tennessee, USA.
Kevin M MotzDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Alexander T HillelDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Funding

Targeting mTOR regulation of T-lymphocytes and fibroblasts in Laryngotracheal StenosisR01DC018567 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI HILLEL, ALEXANDER · 2020 to 2024
$3.8M
E-cadherin deficient epithelium drives airway fibrosis in idiopathic subglottic stenosisR01HL174875 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI Alexander Hillel · 2025 to 2026
$1.5M
Role of E-cadherin in epithelial barrier dysfunction and fibrosis in idiopathic subglottic stenosisR56DC020736 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI HILLEL, ALEXANDER · 2023 to 2023
$154k
NHLBI NIH HHS R01 HL174875NIDCD NIH HHS R01 DC018567NIDCD NIH HHS R56 DC020736
6 · The paper itself

Abstract

objectiveTo assess the effects of localized subglottic knockout of E-cadherin (CDH1 STUDY

designCase-control in vivo mouse study.

settingTertiary care academic hospital.

methodsMice with loxP sites flanking E-cadherin underwent extratracheal placement of a fibrin-plasmin gel embedded with either CRE-expressing or control adenovirus. Mice then underwent chemomechanical injury to induce laryngotracheal stenosis, with harvest of subglottis/tracheas 21 days later. Immunofluorescence and Western blotting were used to confirm E-cadherin knockout. Outcomes of interest included Kaplan-Meier survival curves (n = 40), lamina propria thickness on hematoxylin-eosin (H&E) histology (n = 8), and fibrotic gene expression (n = 3).

resultsImmunofluorescence and Western blotting confirmed decreased E-cadherin expression in CDH1

conclusionCDH1

Indexed as

CadherinsLaryngostenosisAnimalsBlotting, WesternCase-Control StudiesDisease Models, AnimalFibrosisMiceMice, KnockoutCadherinsE‐cadherinepithelial barrieridiopathic subglottic stenosis

Identifiers

PMID40105452
PMCPMC12122222

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.