ArticleMaterials today. Bio2025
Three-dimensional matrix stiffness-based stem cell soil: Tri-phase biomechanical structure promoted human dental pulp stem cells to achieve pulpodentin regeneration.
Article in Materials today. Bio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Mechanical Signaling in the Microenvironment Regulates the Differentiation of Dental Pulp Stem Cells: A Novel Strategy For Pulp-Dentin Complex Regeneration.Stem cell reviews and reports · 2026Review
- The dental pulp as a high-resolution model for neurovascular niche biology.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regeneration of the pulp-dentine complex is characterized by organizational diversity, with both dentine and pulp being essential for regenerating a complete tooth-like structure. Matrix stiffness plays a crucial role in guiding the multi-lineage differentiation of stem cells during the regeneration process. However, human dental pulp stem cell (HDPSCs) differentiation via three-dimensional (3D) matrix stiffness is still ambiguous. This study employed gelatin methacryloyl hydrogels of varying stiffness to investigate their effects on HDPSCs differentiation, and constructing a Tri-Phase Biomechanical Structure. The effects of 3D stiffness on HDPSCs proliferation, morphology, differentiation, and biomineralization were examined. The underlying mechanisms were analyzed by RNA sequencing (RNA-seq). At the same time, the comprehensive effects of 3D matrix stiffness-induced HDPSCs paracrine signals on periapical cells (endothelial cells, macrophages and fibroblasts) were evaluated. In vitro, high stiffness promoted dentin differentiation, medium stiffness supported vascular differentiation, and low stiffness enhanced vascularization of peri-apical cells through paracrine signals. In vivo, treated dentin matrixes implanted in nude mice further confirmed that this Tri-Phase Biomechanical Structure effectively promoted crownward dentin formation, pulp-like regeneration within root canals, and integration with periapical tissues. These findings highlight that understanding HDPSCs responses to 3D matrix stiffness is crucial for guiding targeted, efficient regeneration of a tooth-like pulpodentin complex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.