Evidence map›Paper›PMID 40101838›Full record

ArticleSLAS technology2025

Automation of protein crystallization scaleup via Opentrons-2 liquid handling.

Jacob B DeRoo, Alec A Jones, Caroline K Slaughter, Tim W Ahr, Sam M Stroup, Grace B Thompson, Christopher D Snow

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in SLAS technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jacob B DeRooSchool of Biomedical Engineering, Colorado State University, United States of America.
Alec A JonesSchool of Biomedical Engineering, Colorado State University, United States of America.
Caroline K SlaughterDepartment of Cellular and Molecular Biology, Colorado State University, United States of America.
Tim W AhrDepartment of Chemical Engineering, Colorado State University, United States of America.
Sam M StroupDepartment of Chemistry, Colorado State University, United States of America.
Grace B ThompsonSchool of Biomedical Engineering, Colorado State University, United States of America; Department of Electrical Engineering, Colorado State University, United States of America.
Christopher D SnowSchool of Biomedical Engineering, Colorado State University, United States of America; Department of Cellular and Molecular Biology, Colorado State University, United States of America; Department of Chemical Engineering, Colorado State University, United States of America; Department of Chemistry, Colorado State University, United States of America. Electronic address: christopher.snow@colostate.edu.

Funding

Multiplexed imaging of viral protein processing and assembly in live cellsR01AI168459 · NIAID · COLORADO STATE UNIVERSITY · PI Christopher Davis Snow · 2022 to 2026
$2.3M
NIAID NIH HHS R01 AI168459
6 · The paper itself

Abstract

In this study we present an approach for optimizing protein crystallization trials at the multi-microliter scale utilizing the Opentrons-2 liquid handling robot. Our research demonstrates the robot's capability to automate 24-well sitting drop protein crystallization trials. Using Python scripts for precise control, the study explores the robot's application in mixing and setting up crystallization plates with a model protein (hen egg white lysozyme) and a periplasmic protein from Campylobacter jejuni, a crystal utilized in the Snow lab as a biomaterial for nanotechnology that requires large, consistent batches. In a head-to-head comparison with manual 24-well plate setup, crystal growth statistics indicate our approach can reduce manual labor and increase reliability in protein crystallization, and may also reduce variability, offering an economical and versatile tool for laboratories. This study shows facile adaption of the Opentrons interface and hardware for growth of two different crystal types. All developed liquid handling routines and relevant data files, in addition to demonstration videos are available at https://github.com/jbderoo/Opentrons2-Protein-Crystallization.

Indexed as

Automation, LaboratoryProteinsRoboticsCampylobacter jejuniCrystallizationMuramidasehen egg lysozymeMuramidaseProteinsCrystallographyLiquid handlingProtein crystallizationSitting drop crystallization

Identifiers

PMID40101838
PMCPMC12229254

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.